Prevalence of Genotypes and Subtypes of Gardnerella vaginalis in South African Pregnant Women.

Infectious Diseases in Obstetrics and Gynecology
Kayla PillayNathlee S Abbai

Abstract

Gardnerella vaginalis, a microorganism highly linked to bacterial vaginosis (BV), is understudied in terms of genotypic heterogeneity in South African populations. This study investigated the prevalence of G. vaginalis genotypes in BV-positive, BV-intermediate, and BV-negative South African pregnant women. The study population included n = 354 pregnant women recruited from a public hospital in Durban, South Africa. The women provided self-collected vaginal swabs for BV diagnosis by Nugent scoring. For the genotyping assays, the 16S rRNA and sialidase A genes from BV-negative, BV-intermediate, and BV-positive samples were amplified with G. vaginalis-specific primers. The16S rRNA amplicon was digested with TaqI to generate genotyping profiles, and subtypes were determined by correlating BamHI and HindIII digestion profiles. Phylogenetic analysis was performed on the 16S rRNA and sialidase A sequences. The data analysis was performed with R Statistical Computing software, version 3.6.2. Two different genotypes, GT1 and GT2, were detected. The most prevalent genotype was GT1. Four subtypes (1, 2B, 2AB, and 2C) were shown to be present. The most prevalent subtype was 2B, followed by subtypes 1, 2C, and 2AB. The phylogenetic analysis...Continue Reading

References

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

Molecular Evolutionary Genetics Analysis ( MEGA )
R Statistical Computing
ARDRAhas
DNAMAN
Chromas
ARDRA
Basic Local Alignment Search Tool ( BLAST )

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