Prevalence of Giardia duodenalis genotypes in pre-weaned dairy calves

Veterinary Parasitology
James M TroutRonald Fayer

Abstract

To determine the prevalence of Giardia genotypes in pre-weaned dairy calves, fecal samples were collected from a minimum of 18, 1-7-week-old dairy calves per farm on two farms each in the states of Vermont, New York, Pennsylvania, Maryland, Virginia, North Carolina, and Florida. Samples cleaned of fecal debris and concentrated using CsCl density gradient centrifugation were stained and examined by immunofluorescence microscopy and also subjected to PCR and gene sequence analysis. Prevalence by PCR ranged from 9% on a farm in Pennsylvania to 93% on a farm in Vermont, with an average prevalence for 407 calves on 14 farms of 40%. Gene sequence analysis of the TPI, beta-giardin and 16S rRNA genes revealed 85% of the positive samples to be Assemblage E, while 15% were Assemblage A, although the percentages of these genotypes varied greatly from farm to farm. Some farms had no Assemblage A Giardia. Thus, while a majority of the calves were infected with a genotype that is not known to be infectious for humans, calves on 7 of 14 farms did harbor Assemblage A Giardia. Calves should be considered as a potential source of human infectious cysts in the environment.

References

Jul 1, 1990·The Journal of Infectious Diseases·A BuretM E Olson
Nov 1, 1994·Veterinary Parasitology·L Xiao, R P Herd
Mar 1, 1997·Veterinary Parasitology·M E OlsonT A McAllister
Sep 16, 1999·Molecular Biology and Evolution·P T MonisP L Ey
Apr 27, 2000·Parasitology Today·R C ThompsonW L Homan
Nov 14, 2001·Veterinary Parasitology·R E HuetinkH W Ploeger
Mar 8, 2003·Veterinary Parasitology·A J AppelbeeM E Olson
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Paul T MonisPeter L Ey
Jan 14, 2004·Emerging Infectious Diseases·Irshad M SulaimanLihua Xiao
Jan 1, 1994·Parasitology Today·L Xiao

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Citations

Mar 24, 2012·Folia Parasitologica·Flávio Medeiros Paz e SilvaJoão Pessoa Araújo
Sep 18, 2007·Applied and Environmental Microbiology·Woutrina A MillerEdward R Atwill
Jun 2, 2007·The Journal of Parasitology·M LalleS M Cacciò
Jun 20, 2007·Parasitology·C M Wielinga, R C A Thompson
Jul 17, 2013·International Journal for Parasitology·Una Ryan, Simone M Cacciò
Apr 21, 2005·Parasitology Research·Annunziata GiangasperoDonato Traversa
Mar 14, 2008·Parasitology Research·Yaoyu FengLihua Xiao
Jul 16, 2008·Parasitology Research·P ForondaS Mas-Coma
Apr 20, 2011·Water Research·Robert H BaqueTheresa R Slifko
Dec 9, 2009·Veterinary Parasitology·Marianne LebbadStaffan G Svärd
Mar 17, 2009·Experimental Parasitology·Thomas GeurdenEdwin Claerebout
Mar 7, 2009·Veterinary Parasitology·Mónica SantínRonald Fayer
Dec 16, 2006·The Journal of Eukaryotic Microbiology·André A AlmeidaJosé M Correia Da Costa
Aug 15, 2006·Veterinary Parasitology·Fabienne D UehlingerRyan M O'Handley
May 2, 2006·Veterinary Parasitology·Inger Sofie HamnesLucy Robertson
May 2, 2006·Veterinary Parasitology·James M TroutRonald Fayer
May 17, 2006·International Journal for Parasitology·J W B van der GiessenT G Mank
Jun 1, 2005·Veterinary Parasitology·James M TroutRonald Fayer
Jul 28, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Harshanie AbeywardenaRobin B Gasser
Jun 15, 2007·Veterinary Parasitology·James M TroutRonald Fayer
Oct 26, 2010·Veterinary Parasitology·Relja BeckSimone M Cacciò
Oct 14, 2011·Veterinary Parasitology·Miguella P Mark-CarewHussni O Mohammed
Mar 12, 2013·Veterinary Parasitology·Mónica SantínRonald Fayer
Sep 18, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Harshanie AbeywardenaRobin B Gasser
May 27, 2008·Molecular and Biochemical Parasitology·Simone M Cacciò, Una Ryan
Jan 21, 2012·Research in Veterinary Science·María Teresa Gómez-MuñozRonald Fayer

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