Prevalence of HCV NS3 pre-treatment resistance associated amino acid variants within a Scottish cohort

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Samantha J ShepherdRory N Gunson

Abstract

Protease inhibitors (PI) including boceprevir, telaprevir and simeprevir have revolutionised HCV genotype 1 treatment since their introduction. A number of pre-treatment resistance associated amino acid variants (RAVs) and polymorphisms have been associated with reduced response to treatment. We measured the prevalence of RAVs/polymorphisms in a PI treatment-naïve HCV genotype 1 Scottish cohort using Sanger sequencing. Chronically infected, treatment-naïve, HCV genotype 1 patients (n = 146) attending NHS Greater Glasgow and Clyde clinics were investigated for RAVs/polymorphisms to the PIs boceprevir, telaprevir and simeprevir. The NS3/4A region was amplified by nested polymerase chain reaction. The 1.4 kb amplified product was sequenced using an ABI 3710XL DNA sequencer. Sequence analysis was performed using web-based ReCall (beta 2.10). Amino acid positions 36, 41, 43, 54, 55, 80, 109, 122, 155, 156, 168 and 170 were analysed for RAVs/polymorphisms. Overall, 23.29% (34/146) of patients had an RAV or polymorphism detected. Overall, 13.69% (20/146) of patients had HCV virus that contained the Q8 K polymorphism. Other RAVs detected were: V36 M 0.70% (1/146), V36L 0.70% (1/146), T54S 6.85% (10/146), V55A 3.42% (5/146) and V/I170A ...Continue Reading

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Citations

Dec 19, 2015·The Journal of Antimicrobial Chemotherapy·V C Di MaioUNKNOWN HCV Italian Resistance Network Study Group
Dec 17, 2015·Virology Journal·Kattareeya Kumthip, Niwat Maneekarn
Apr 6, 2016·Clinical and Molecular Hepatology·UNKNOWN Korean Association for the Study of the Liver
Jun 25, 2016·Viruses·Ahmed Atef MesalamPhilip Meuleman
Sep 30, 2016·PloS One·María Ángeles Jimenez-SousaSalvador Resino
Mar 5, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Akihiro TamoriNorifumi Kawada
Jun 28, 2019·Singapore Medical Journal·Benjy Yi-Min SohWan Cheng Chow
Sep 10, 2020·Virusdisease·Ana Elisa de Figueiredo Miranda MundimAntonio Márcio Teodoro Cordeiro Silva

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Methods Mentioned

BETA
PCR

Software Mentioned

ReCall

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