Prevalence of high serotonin uptake in lymphocytes of abstinent alcoholics

Biochemical Pharmacology
B A FarajR T Jackson

Abstract

An impairment in serotonergic neurotransmission may be associated with alcoholism. We recently identified a high-affinity serotonin transporter (5-HTT) in human peripheral blood lymphocytes (PBLs). Moreover, molecular analysis of RNA samples of human lymphocytes using reverse transcription, coupled with polymerase chain reaction, enabled us to confirm the expression of a 5-HTT identical to the one reported in neuronal tissues, as evidenced by hybridization and sequence analysis. In this investigation, we measured the serotonin (5-HT) uptake in PBLs of recovering alcoholics (N = 10) with long-term abstinence (2-10 years) and non-alcoholic controls (N = 10). 5-HT uptake was measured by incubating 1 x 10(7) cells of PBLs with [3H]5-HT (3-1000 nM; sp. act. 23 Ci/mmol) for 10 min at 37 degrees. The results of this preliminary study revealed that abstinent alcoholics had significantly (P < 0.01) increased uptake of 5-HT (43.6 +/- 5.70 pmol/10(7) cells) as compared with controls (23.33 +/- 2.50 pmol/10(7) cells). An enhanced uptake of 5-HT in PBLs of abstinent alcoholics agrees with previously reported observations of increased 5-HT uptake in brain and platelets of former alcoholics and their descendants. This suggested that a seroton...Continue Reading

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Citations

Sep 23, 2003·Progress in Neuro-psychopharmacology & Biological Psychiatry·Gustavo E Tafet, Renato Bernardini
Mar 31, 2011·Pharmacology & Therapeutics·Lynette C Daws, Georgianna G Gould
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Oct 25, 2000·Alcoholism, Clinical and Experimental Research·B A Johnson
Nov 24, 2004·Life Sciences·Nela PivacDragica Kozarić-Kovacić

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