Prevalence of human immunodeficiency virus type 1 (HIV-1) non-B subtypes in foreigners living in Madrid, Spain, and comparison of the performances of the AMPLICOR HIV-1 MONITOR version 1.0 and the new automated version 1.5

Journal of Clinical Microbiology
A HolguínV Soriano

Abstract

Plasma specimens collected in 1999 from 32 human immunodeficiency virus type 1 (HIV-1)-infected foreigners living in Madrid, Spain, were examined for the presence of non-B subtypes. Furthermore, plasma viremia was quantified using two different AMPLICOR HIV-1 MONITOR tests, version 1.0 and the new upgraded and automated version 1.5 (COBAS). Most patients came from Africa, where they most likely had acquired HIV-1 infection through sexual contact. HIV-1 genetic subtyping was based on the phylogenetic analysis of the protease gene. Twenty-two subtype B, six subtype G, two subtype C, one subtype A, and one D subtype infection were found. Overall, non-B subtypes represented 31.25% of the study population. Irrespective of the HIV-1 variant, viral load values above the detection limit (200 HIV RNA copies/ml) increased from 56.2 to 71.9% for results obtained using MONITOR version 1.0 and COBAS, respectively. Moreover, significant differences in viral load values (>0.5 logs) were recognized in up to 37.5% of samples. In summary, COBAS seemed to be more reliable for testing plasma viral load in HIV-infected immigrants living in Spain, one third of whom carried non-B subtypes.

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Citations

Feb 14, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Rami Kantor, David Katzenstein
Feb 25, 2003·AIDS Research and Human Retroviruses·Herve FleuryUNKNOWN Agence Nationale de Recherche sur le SIDA AC11 Laboratory Network
Jan 31, 2002·AIDS·I D TattJ P Clewley
Dec 4, 2002·AIDS·Sabrina DerooJean-Claude Schmit
Aug 17, 2002·The Pediatric Infectious Disease Journal·Michael D Nissen, Theo P Sloots
Sep 15, 2005·HIV Medicine·E LospitaoA Holguín
Mar 2, 2002·Sexually Transmitted Infections·J C MartínV Soriano
Jul 23, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Melvyn SmithMark Zuckerman
May 18, 2004·Infectious Disease Clinics of North America·Cristian ApetreiStephen M Smith
Oct 7, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Africa HolguínVincent Soriano
Jul 13, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Iain D TattJohn V Parry
Jan 14, 2005·Journal of Medical Virology·Africa HolguínVincent Soriano
Jun 7, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jean-Christophe PlantierFrançois Simon

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