Prevalence of human papillomavirus genotypes among women with cervical cancer in Ghana

Infectious Agents and Cancer
A K AwuaEdwin Wiredu

Abstract

Human Papillomavirus (HPV) infections have been shown to be a necessary risk factor for the development of cervical cancer. However, HPV genotype distribution varies geographically, both in type and relative prevalence. In order to ensure a successful introduction of available vaccines, there is the need to identify pre-vaccination HPV genotype prevalence in Ghana and the extent of single and multiple-infections. Paraffin-embedded cervical tissues of 256 confirmed cervical cancer cases diagnosed at the Korle-Bu Teaching Hospital during the period January 2004 to December 2006 were selected after hematoxylin and eosin staining and confirmation. Following a heat-proteinase K-based tissue lysis, HPV was detected and typed by a nested-multiplex PCR assay using an E6/E7 consensus primer and type-specific primers. Of the 256 cases, 230 (89.8 %, 95 % CI 85.7-93.4 %) were positive for HPV DNA. HPV18 (47.4 %), HPV59 (42.2 %), HPV45 (37.4 %) and HPV16 (9.0 %) were the four common HPV genotypes detected. A total of 110 (47.8 %) of the 230 HPV DNA positive tissues, were infected by a single HPV genotype while the other 120 (52.2 %) were infected by multiple HPV genotypes. A significant association was determined between each of the followi...Continue Reading

References

Dec 16, 1997·International Journal of Cancer. Journal International Du Cancer·K L LiawC J Chen
May 9, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E R UngerW C Reeves
Oct 2, 2001·CA: a Cancer Journal for Clinicians·M F Janicek, H E Averette
Nov 14, 2001·Lancet·X CastellsaguéP Alonso
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
Jun 17, 2003·Journal of the National Cancer Institute. Monographs·F Xavier Bosch, Silvia de Sanjosé
Sep 6, 2003·Journal of Clinical Microbiology·Philippe MayaudDavid C W Mabey
Jan 13, 2004·Journal of Clinical Microbiology·Hsing-Pei LinJau-Tsuen Kao
Mar 9, 2004·Journal of Clinical Microbiology·M Paz CañadasSilvia de Sanjosé
Jul 10, 2004·Journal of Clinical Microbiology·K SotlarB Bültmann
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
Apr 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kaiyo TakuboTomio Arai
Jun 22, 2006·BMC Public Health·Edwin K Wiredu, Henry B Armah
Jul 17, 2009·The New England Journal of Medicine·Jessica A Kahn
Aug 19, 2010·BMC Infectious Diseases·Georgette Adjorlolo-JohnsonTerence L Chorba
Nov 15, 2011·Virology Journal·Franca PirasPaola Sirigu
Nov 16, 2011·BMC Infectious Diseases·Paloma MartínCarmen Bellas
Aug 10, 2013·International Journal of Cancer. Journal International Du Cancer·Lynette DennyJohannes Schmidt
Nov 1, 2006·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·F Xavier BoschXavier Castellsagué

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Citations

Jul 22, 2017·Cytopathology : Official Journal of the British Society for Clinical Cytology·G Ramos RiveraA S Fox
Jan 16, 2018·Health Care for Women International·Michelle S WilliamsKevin Fontaine
Dec 22, 2017·Journal of Cell Communication and Signaling·Shideh Kazerounian, Jack Lawler
Aug 14, 2019·Reviews in Medical Virology·Fateme TaheriEbrahim Faghihloo
Nov 4, 2016·Medical Microbiology and Immunology·Marco H SchulzeSteffi Silling
Jul 6, 2018·Journal of Cancer Education : the Official Journal of the American Association for Cancer Education·Michelle S WilliamsKevin Fontaine
Jan 15, 2020·Analytical Cellular Pathology (Amsterdam)·Xiaoqin CaoXibin Sun
Apr 14, 2021·BMC Women's Health·Evans Appiah OseiEzekiel Oti-Boadi
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xin Liu

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Methods Mentioned

BETA
PCR
genotyping
PCRs
electrophoresis
biopsy

Software Mentioned

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