Prevalence of neutralizing antibodies against liver-tropic adeno-associated virus serotype vectors in 100 healthy Chinese and its potential relation to body constitutions

Journal of Integrative Medicine
Chen LingChang-quan Ling

Abstract

Recombinant adeno-associated virus (rAAV) serotype 2, 3 and 8 vectors are the most promising liver-tropic AAV serotype vectors. Liver diseases are significant problems in China. However, to date, few studies on AAV neutralizing antibodies (Nabs) were working with the Chinese population or with the rAAV3 vectors. The present study aimed to determine the prevalence of Nabs in Chinese population against wild-type AAV2, AAV3 and AAV8 capsids as well as additional two AAV3 variants. In addition, we performed a preliminary analysis to investigate the potential influence of traditional Chinese medicine body constitutions on AAV Nabs. Our work demonstrated that the majority of healthy Chinese subjects were positive for AAV Nabs, with the order of AAV2>AAV3=AAVLK03>AAV8. There was no difference between: 1) AAV3 and its variants; 2) male and female subjects; and 3) different age cohorts (≤35, 36-50, and ≥51 years old). People in the Qi-deficiency constitution had significantly increased AAV8 Nabs than people in the Gentleness constitution. Our studies may have impact on the future clinical design of AAV-based gene therapy in the Chinese population.

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Citations

Sep 26, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shaoyong LiGuangping Gao
May 21, 2016·Molecular Therapy. Methods & Clinical Development·Chen LingArun Srivastava
Jun 18, 2016·Human Gene Therapy. Clinical Development·Roberto Calcedo, James M Wilson
Jul 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tianzhuo YaoDemin Zhou
Jul 22, 2018·Human Gene Therapy·Dany P PerocheauJulien Baruteau
Jul 5, 2017·Experimental and Therapeutic Medicine·Zhongye WangXiulan Du
Jan 12, 2021·Molecular Therapy. Methods & Clinical Development·Randolph QianXiao Xiao
Apr 25, 2021·Virology Journal·Rossana Colón-ThilletDaniel Stone
May 1, 2021·Frontiers in Immunology·Hildegund C J Ertl
Sep 22, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Min LiChen Ling
Dec 5, 2021·Human Gene Therapy·Arpana KhatriSuryanarayan Somanathan

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