Prevalence of serogroups and virulence factors of Escherichia coli strains isolated from pigs with postweaning diarrhoea in eastern China

Veterinary Microbiology
Xiang ChenXiu Fan Liu

Abstract

This study was undertaken to determine the present distribution of serogroups, hemolytic activity and virulence factors among Escherichia coli strains isolated from pigs with postweaning diarrhoea from eight provinces in eastern China. Two hundred and fifteen E. coli isolates were serogrouped with O-antisera, investigated for hemolytic activity, assessed for F4, F5, F6, F18 and F41 fimbrial antigens by monoclonal antibodies and detected for genes of enterotoxins and shiga-toxin-two-variant (Stx2e) by a multiplex polymerase chain reaction (PCR). Among these E. coli isolates, 140 were determined to be placed in serogroups, 52 were unable to be serogrouped and the rest 23 auto-agglutinated. These isolates distributed in 45 serogroups and 64.3% (90/140) belonged to 12 O serogroups: O8, O9, O11, O20, O32, O91, O93, O101, O107, O115, O116 and O131. Hemolytic activity was detected in 11.6% (25/215) of all isolates. Several uncommon O serogroups were discovered in this study. Agglutination tests showed that 50.2% (108/215) of these isolates were positive for one or more of the five fimbrial antigens. Seventy-two E. coli strains expressed single fimbria and 36 strains expressed two or more fimbriae. Among these 215 E. coli isolates, str...Continue Reading

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Citations

Oct 17, 2012·Veterinary Immunology and Immunopathology·Vesna MelkebeekEric Cox
Jul 6, 2010·Developmental and Comparative Immunology·Bert DevriendtEric Cox
Mar 5, 2016·Animal : an International Journal of Animal Bioscience·H Y JiL S Huang
May 2, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Chitrita DebRoyBhushan M Jayarao
Dec 7, 2017·Frontiers in Microbiology·Sabine DelannoyIsabelle Kempf
Feb 19, 2013·Tropical Animal Health and Production·Ramadimetja Prescilla MohlatloleEdgar Farai Dzomba
Mar 31, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Myrthe S GilbertSaskia W C van Mil
Oct 17, 2020·Frontiers in Veterinary Science·Valentina BaldoGiovanni Loris Alborali
Sep 20, 2020·Applied and Environmental Microbiology·Vanesa GarcíaAna Herrero-Fresno

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