Prevalence of serum antibodies to Helicobacter pylori VacA and CagA and gastric diseases in Chile

Journal of Medical Microbiology
G FigueroaR Acuña

Abstract

The objective of this study was to evaluate the prevalence of antibodies to Helicobacter pylori CagA and VacA proteins and correlate this prevalence with gastric diseases in colonised Chileans. The study was performed in 418 adults colonised with H. pylori: 316 with gastroduodenal pathology (152 duodenal ulcer, 14 gastric cancer and 150 gastritis patients) and 102 asymptomatic subjects. Serum IgG antibodies to H. pylori were determined by enzyme immunoassay (EIA). Antibodies to VacA and CagA proteins were detected by Western blotting. In a subgroup of the patients, the vacuolating activity was determined by HeLa cell assay and the CagA product was confirmed by PCR assay. IgG antibodies to both VacA and CagA proteins of H. pylori were found in 270 (85%) of 316 colonised gastric patients and in 72 (71%) of 102 asymptomatic subjects. Colonisation with virulent strains was significantly higher among duodenal ulcer and gastric cancer patients than in gastritis patients or asymptomatic subjects. Infections with VacA+/ CagA+ H. pylori strains is common in Chile but, in contrast to some Asian countries, this phenotype was more prevalent in isolates from patients with more severe gastric pathologies.

Citations

Dec 17, 2008·Scandinavian Journal of Infectious Diseases·Abdallah Said EssaHany Said Sabry
Jul 23, 2011·Cancer Causes & Control : CCC·Guadalupe AyalaJavier Torres
May 18, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Najmeh KaramiMarjan Mohammadi
Sep 3, 2002·Clinical and Diagnostic Laboratory Immunology·G FigueroaM S Toledo
Feb 6, 2013·Gut Microbes·Dacie R Bridge, D Scott Merrell
Sep 5, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Margarita Camorlinga-PonceJavier Torres
Apr 19, 2003·Journal of Clinical Microbiology·Adriana SantosFrancis Mégraud

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