Prevalence of temperature-dependent heat capacity changes in protein-DNA interactions

Biophysical Journal
Chin-Chi LiuVince J LiCata

Abstract

A large, negative DeltaCp of DNA binding is a thermodynamic property of the majority of sequence-specific DNA-protein interactions, and a common, but not universal property of non-sequence-specific DNA binding. In a recent study of the binding of Taq polymerase to DNA, we showed that both the full-length polymerase and its "Klentaq" large fragment bind to primed-template DNA with significant negative heat capacities. Herein, we have extended this analysis by analyzing this data for temperature-variable heat capacity effects (DeltaDeltaCp), and have similarly analyzed an additional 47 protein-DNA binding pairs from the scientific literature. Over half of the systems examined can be easily fit to a function that includes a DeltaDeltaCp parameter. Of these, 90% display negative DeltaDeltaCp values, with the result that the DeltaCp of DNA binding will become more negative with rising temperature. The results of this collective analysis have potentially significant consequences for current quantitative theories relating DeltaCp values to changes in accessible surface area, which rely on the assumption of temperature invariance of the DeltaCp of binding. Solution structural data for Klentaq polymerase demonstrate that the observed he...Continue Reading

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J M Sturtevant
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y TakedaC P Mudd
Oct 20, 1989·Journal of Molecular Biology·J H HaM T Record
Oct 1, 1995·Protein Science : a Publication of the Protein Society·J K MyersJ M Scholtz
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Lundbäck, T Härd
Oct 1, 1996·Protein Science : a Publication of the Protein Society·C J Morton, J E Ladbury
May 2, 1998·Biochemistry·A G K-unneR K Allemann
Aug 5, 1997·Chemical Reviews·Andrew D. Robertson, Kenneth P. Murphy
Sep 23, 2003·Nucleic Acids Research·Kausiki Datta, Vince J LiCata
Apr 21, 2004·Journal of Molecular Biology·Simon BergqvistJohn E Ladbury
Sep 29, 2004·Journal of Molecular Biology·William B PetersJohn W Shriver
Sep 29, 2004·Journal of Molecular Biology·Anatoly I DraganPeter L Privalov
Dec 13, 2005·Biophysical Journal·Kausiki DattaVince J LiCata

❮ Previous
Next ❯

Citations

Jul 8, 2009·International Journal of Molecular Sciences·Lina BaranauskieneDaumantas Matulis
Oct 18, 2015·Biochimica Et Biophysica Acta·S VegaA Velazquez-Campoy
Mar 10, 2010·Journal of Molecular Recognition : JMR·Robert J FalconerBrett M Collins
Oct 7, 2010·Journal of Receptor and Signal Transduction Research·Vladimir Pliska
Sep 10, 2020·The Journal of Cell Biology·Colin R Gliech, Andrew J Holland
Feb 17, 2019·Nature Communications·Valérie GuilletLionel Mourey
Jun 27, 2020·Angewandte Chemie·Katharina AchaziGerd Multhaup
Mar 31, 2021·PloS One·Lynn W DeLeeuwJonathan B Chaires
Aug 11, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xiao XuMatthias Ballauff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.