Prevalence of viable Chlamydia pneumoniae in peripheral blood mononuclear cells of healthy blood donors

Transfusion
Hiroyuki YamaguchiShigeru Kamiya

Abstract

Demonstration of viable Chlamydia (Chlamydophila) pneumoniae in peripheral blood mononuclear cells (PBMNCs) is essential to understand the involvement of C. pneumoniae in atherosclerosis. Nevertheless, the prevalence of viable C. pneumoniae in the blood of healthy donors has not yet been studied. The presence of C. pneumoniae transcript in PBMNCs from blood of healthy human donors was assessed by real-time reverse transcription-polymerase chain reaction (RT-PCR) with primers for C. pneumoniae 16S rRNA, which is more sensitive than genomic-DNA-based analysis, and by the use of staining with fluorescein isothiocyanate-conjugated chlamydia monoclonal antibody (MoAb). Thirteen of 70 donors (18.5%) showed the presence of bacterial transcript in cultured PBMNCs. The prevalence of bacterial detection and bacterial numbers was significantly increased in PBMNC cultures incubated with cycloheximide. Immunostaining of PBMNCs with antichlamydial MoAb also revealed the presence of bacterial antigen in the PBMNCs judged as positive. Nevertheless, cultivation of C. pneumoniae from all PCR-positive donors was unsuccessful. There was no significant correlation between the presence of chlamydia and either sex or current smoking habits. A possibl...Continue Reading

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Citations

Sep 8, 2005·Journal of Clinical Microbiology·Hideaki IkejimaYoshimasa Yamamoto
Apr 21, 2006·International Journal of Experimental Pathology·Hiroyuki YamaguchiShigeru Kamiya
Sep 26, 2006·Trends in Microbiology·Charles W Stratton, David B Wheldon
Aug 4, 2012·Journal of Medical Microbiology·Antonietta RizzoCaterina Romano Carratelli
Feb 24, 2015·International Journal of Hygiene and Environmental Health·A PullieroA Izzotti
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May 30, 2019·Frontiers in Cellular and Infection Microbiology·Diego J CastilloMarnie Potgieter
Jul 20, 2021·Infection and Immunity·Zengzi ZhouGuangming Zhong

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