Prevascularization of dermal substitutes with adipose tissue-derived microvascular fragments enhances early skin grafting

Scientific Reports
Florian S FruehM W Laschke

Abstract

Split-thickness skin grafts (STSG) are still the gold standard for the treatment of most skin defects. Hence, there is an ongoing need to improve this procedure. For this purpose, we herein analyzed dermal matrices seeded with adipose tissue-derived microvascular fragments (ad-MVF) in a bradythrophic wound model. In additional experiments, the matrices were covered with autologous STSG 10 days after implantation. Green fluorescence protein (GFP)+ ad-MVF were isolated from C57BL/6-Tg(CAG-EGFP)1Osb/J mice and seeded onto collagen-glycosaminoglycan matrices. Non-seeded and prevascularized matrices were implanted into full-thickness skin defects on the skull of CD1 nu/nu mice for 21 days. Vascularization, lymphangiogenesis and incorporation of the matrices were analyzed using photo-acoustic imaging, trans-illumination stereomicroscopy, histology, and immunohistochemistry. The survival rate of STSG was assessed by planimetry. After 21 days, the density of microvascular and lymphatic networks was significantly higher in prevascularized matrices when compared to controls. This was associated with an improved implant integration. Moreover, prevascularization with ad-MVF allowed successful autologous skin grafting already at day 10, whi...Continue Reading

References

May 5, 1997·FEBS Letters·M OkabeY Nishimune
Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·M Skobe, M Detmar
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·I KimT A Mustoe
Apr 27, 2002·Science·Julie JamesonWendy L Havran
Nov 19, 2003·Advanced Drug Delivery Reviews·Zbigniew Ruszczak
Sep 30, 2004·Journal of the American College of Surgeons·Russell R ReidThomas A Mustoe
Jul 23, 2008·Journal of Biomaterials Science. Polymer Edition·Robert C Fang, Thomas A Mustoe
Dec 4, 2008·Plastic and Reconstructive Surgery·Nicole LindenblattBrigitte Vollmar
Mar 25, 2009·The Journal of Experimental Medicine·Antoine ToulonWendy L Havran
Jun 26, 2009·Tissue Engineering. Part B, Reviews·Matthias W LaschkeMichael D Menger
Oct 30, 2009·Journal of the Royal Society, Interface·Rostislav V ShevchenkoS Elizabeth James
Nov 12, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chandan K SenMichael T Longaker
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wendy L Havran, Julie M Jameson
Jul 3, 2010·Plastic and Reconstructive Surgery·Nicole LindenblattMaurizio Calcagni
Dec 1, 2012·Stem Cells Translational Medicine·Aleksander SkardalShay Soker
Jun 4, 2013·Nature Medicine·Denise GayGeorge Cotsarelis
Jan 31, 2014·Science Translational Medicine·Daniela MarinoErnst Reichmann
Mar 22, 2014·Acta Biomaterialia·M T CerqueiraA P Marques
Jun 28, 2014·The Journal of Surgical Research·Jennifer S McDanielChristopher R Rathbone
Sep 17, 2014·Journal of Visualized Experiments : JoVE·Thilo L SchenckJosé T Egaña
Dec 5, 2014·Science Translational Medicine·Sabine A EmingMarjana Tomic-Canic
Feb 13, 2015·Plastic and Reconstructive Surgery. Global Open·Heidi DebelsWayne Morrison
Jul 4, 2015·Trends in Biotechnology·Matthias W Laschke, Michael D Menger
Dec 18, 2015·Biotechnology Advances·Matthias W Laschke, Michael D Menger
Mar 29, 2016·Journal of Cellular Biochemistry·S StrassburgG B Stark
Jul 22, 2016·Critical Reviews in Biotechnology·Florian S FruehMatthias W Laschke
Aug 25, 2016·Plastic and Reconstructive Surgery·Mayer Tenenhaus, Hans-Oliver Rennekampff
Mar 6, 2017·The Journal of Investigative Dermatology·Lucília Pereira da SilvaAlexandra Pinto Marques
May 19, 2017·Journal of Visualized Experiments : JoVE·Florian S FruehMatthias W Laschke
Feb 20, 2018·European Journal of Immunology·Eric HaertelSabine Werner

❮ Previous
Next ❯

Citations

Jan 17, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marissa A RuehleNick J Willett
Oct 11, 2019·Journal of Wound Care·Ferrán Plá SánchezFrancisco Javier Serrano Hernando
Aug 1, 2020·Advanced Healthcare Materials·Kevin T Campbell, Eduardo A Silva
Apr 4, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Amita AjitLissy K Krishnan
Jul 30, 2019·Stem Cells International·Amtoj KaurSujata Mohanty
Jul 29, 2020·International Journal of Molecular Sciences·Elena GroppaSerena Zacchigna
Sep 15, 2020·Dose-response : a Publication of International Hormesis Society·Cuihua LiuBing Wang
Nov 6, 2020·International Journal of Molecular Sciences·Wasima Oualla-BachiriSalvador Arias-Santiago
Aug 14, 2021·Tissue Engineering and Regenerative Medicine·Surasak KasetsirikuYufeng Zhou
Sep 19, 2021·Tissue Engineering and Regenerative Medicine·Thomas SpäterMatthias W Laschke
Nov 4, 2021·Tissue Engineering. Part B, Reviews·Matthias W Laschke, Michael D Menger

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
Electrophoresis
biopsy

Software Mentioned

cellSens Dimension
Systat
Integra
SigmaPlot
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.