Prevention of autoimmune diabetes and induction of β-cell proliferation in NOD mice by hyperbaric oxygen therapy.

Diabetes
Gaetano FaleoAntonello Pileggi

Abstract

We evaluated the effects of hyperbaric oxygen therapy (HOT) on autoimmune diabetes development in nonobese diabetic (NOD) mice. Animals received no treatment or daily 60-min HOT 100% oxygen (HOT-100%) at 2.0 atmospheres absolute and were monitored for diabetes onset, insulitis, infiltrating cells, immune cell function, and β-cell apoptosis and proliferation. Cyclophosphamide-induced diabetes onset was reduced from 85.3% in controls to 48% after HOT-100% (P < 0.005) and paralleled by lower insulitis. Spontaneous diabetes incidence reduced from 85% in controls to 65% in HOT-100% (P = 0.01). Prediabetic mice receiving HOT-100% showed lower insulitis scores, reduced T-cell proliferation upon stimulation in vitro (P < 0.03), increased CD62L expression in T cells (P < 0.04), reduced costimulation markers (CD40, DC80, and CD86), and reduced major histocompatibility complex class II expression in dendritic cells (DCs) (P < 0.025), compared with controls. After autoimmunity was established, HOT was less effective. HOT-100% yielded reduced apoptosis (transferase-mediated dUTP nick-end labeling-positive insulin-positive cells; P < 0.01) and increased proliferation (bromodeoxyuridine incorporation; P < 0.001) of insulin-positive cells comp...Continue Reading

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Citations

Feb 26, 2014·The Journal of Experimental Medicine·Jason MiskaZhibin Chen
Jun 9, 2014·Medical Hypotheses·Xiaojing YeGang Zhou
Nov 28, 2013·Journal of Vascular Research·Ines Drenjancevic, Aleksandar Kibel
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Methods Mentioned

BETA
flow cytometry
Assay
light microscopy
fluorescence microscopy
confocal microscopy
Flow

Software Mentioned

FACSDiva
Prism
Leica Application Suite
GraphPad

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