PMID: 11934229Apr 6, 2002Paper

Prevention of autoimmune insulitis by delivery of interleukin-4 plasmid using a soluble and biodegradable polymeric carrier

Pharmaceutical Research
Minhyung LeeSung Wan Ki

Abstract

We delivered interleukin-4 (IL-4) plasmid (pCAGGS-IL-4) using the biodegradable polymer, poly[alpha-(4-aminobutyl)-L-glycolic acid] (PAGA), to prevent autoimmune insulitis in NOD mice. The pCAGGS-IL-4/PAGA complex was transfected to 293T cells. The expression level of IL-4 was measured by ELISA. The pCAGGS IL-4/PAGA complex was injected once to NOD mice intravenously at the age of 4 weeks. RT-PCR was performed to evaluate the level of the IL-4 mRNA in the liver. At 6 weeks after the injection, the grade of insulitis of the mice was evaluated by double blind methods. In vitro transfecton assays showed that PAGA enhanced the expression of IL-4 in 293T cells. RT-PCR of the liver showed that IL-4 was expressed highest in the complex injected group. In the plasmid/PAGA complex injected group, the prevalence of severe insulitis in NOD mice was markedly improved, suggesting that PAGA enhanced the delivery of IL-4 plasmid. The pCAGGS-IL-4/PAGA complex is an effective system to prevent autoimmune insulitis in NOD mice and applicable for the prevention of autoimmune diabetes.

Citations

Dec 2, 2005·International Reviews of Immunology·Roy EldorItamar Raz
Nov 5, 2014·Expert Opinion on Drug Delivery·Thanh-Huyen Tran, Mansoor M Amiji
Jun 13, 2003·International Journal of Experimental Pathology·T HayashiT Onodera
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May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Yu-Chuan LiuC Ted Lee
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