Prevention of ESKAPE pathogen biofilm formation by antimicrobial peptides WLBU2 and LL37

International Journal of Antimicrobial Agents
Qiao LinY P Di

Abstract

Bacterial biofilm-dependent infections (e.g. cystic fibrosis, surgical sites, and medical implants) are associated with enhanced drug-resistance and are thus difficult to eradicate. The goal of this study was to systematically compare three distinct classes of antimicrobial peptides (AMPs) that include the clinically used antibiotic colistin, the natural AMP LL37, the engineered cationic-AMP WLBU2, and four commonly used antibiotics with different bactericidal mechanisms (tobramycin, ciprofloxacin, ceftazidime, and vancomycin) for biofilm prevention properties. Using biofilm-prevention assays, we detected bacterial biomass post-attachment in subinhibitory concentrations (1/3 of the minimum inhibitory concentration [MIC]) for each AMP by the crystal violet method, to distinguish the commonly known bactericidal activity from potentially distinct mechanisms of biofilm prevention. Biofilm regulatory gene expression was assessed using RT-qPCR for correlation with biofilm growth inhibition. Commonly used antibiotics at 1x MIC showed modest ESKAPE biofilm prevention while 1/3 MIC of AMPs demonstrated up to 90% biofilm prevention. WLBU2 was generally more effective in preventing bacterial attachment than colistin and LL37. Changes in b...Continue Reading

Citations

Jun 20, 2019·Journal of Medical Microbiology·Lucilla DolzaniMonica Benincasa
Jun 3, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jonathan B MandellKenneth L Urish
May 18, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Na ZhanAnshan Shan
Feb 20, 2019·Frontiers in Chemistry·Evan F HaneyRobert E W Hancock
Jun 19, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yuanna ShenHaiyan Hu
Apr 17, 2019·Frontiers in Microbiology·Mansura S MulaniKarishma R Pardesi
Aug 30, 2020·International Journal of Molecular Sciences·Ana GomesPaula Gomes
Jan 22, 2021·Applied Microbiology and Biotechnology·Jing LiHuancai Lin
Sep 2, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gopal Ramamourthy, Hans J Vogel
Feb 3, 2021·ACS Biomaterials Science & Engineering·Qiao LinYuan-Pu Peter Di
May 15, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Dandan LiShuaibo Dong
May 25, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ruifang LiLiang Huang
Jun 3, 2021·Antibiotics·Kylen E Ridyard, Joerg Overhage
Mar 16, 2021·Terapevticheskiĭ arkhiv·N I GabrielyanV М Zakharevich

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.