Prevention of glomerular crescent formation in glomerulonephritis by mycophenolate mofetil in rats

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Shin-Ichi TakedaEiji Kobayashi

Abstract

Glomerular crescent formation is a prominent feature of aggressive forms of glomerulonephritis (GN) and is associated with a poor prognosis. We investigated whether the potent immunosuppressive agent mycophenolate mofetil (MMF) could prevent crescent formation in a model of anti-glomerular basement membrane (GBM) GN in the rat. GN with glomerular crescents was induced by the injection of anti-GBM antibody to female Wistar-Kyoto (WKY/NCrj) rats. The experimental rats were divided into two groups: rats received vehicle (0.5% carboxymethylcerlose) or MMF (20 mg/kg/day) orally. Body weight was measured and the urine and blood samples were evaluated. The rats were sacrificed at day 14, and histological analysis was performed. The mRNA expression of cytokines and adhesion molecules in the kidney was analysed by reverse transcription-polymerase chain reaction (RT-PCR). Marked proteinuria, glomerular crescent formation and glomerulosclerosis were observed in this model, and these were significantly reduced by MMF treatment. Marked glomerular macrophage and T-cell infiltration was also observed, and MMF treatment significantly inhibited macrophage but not T-cell infiltration. RT-PCR and immunohistochemical analysis revealed that mRNA an...Continue Reading

References

Oct 1, 1992·The Journal of Pathology·Y SadoT Okigaki
Oct 1, 1995·Journal of the American Society of Nephrology : JASN·R H PichlerW G Couser
Nov 1, 1996·Journal of the American Society of Nephrology : JASN·R C AtkinsH Y Lan
Feb 7, 1998·Kidney International·H Y LanR C Atkins
Nov 10, 1998·Journal of the American Society of Nephrology : JASN·R ZiswilerH P Marti
Mar 3, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·I A HauserM Goppelt-Struebe
Mar 11, 2000·The Journal of Clinical Investigation·J ReynoldsC D Pusey
Jul 6, 2000·Immunopharmacology·T S Mele, P F Halloran
Aug 31, 2000·Journal of the American Society of Nephrology : JASN·John Michael BonviniRudolf P Wüthrich
Dec 1, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D J Nikolic-Paterson
Feb 19, 2002·Kidney International·Michael J ChoiWilliam A Briggs
Mar 26, 2002·Journal of the American Society of Nephrology : JASN·Elena NietoFrancisco Mampaso
Jun 29, 2002·Journal of the American Society of Nephrology : JASN·Matthias KretzlerHermann-Josef Gröne
Dec 11, 2002·Kidney International·Ricardo UtimuraIrene De Lourdes Noronha

❮ Previous
Next ❯

Citations

Oct 26, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Shin-ichi TakedaEiji Kusano
Jan 15, 2014·Clinical Kidney Journal·Anna BjerreChristina Dörje
Oct 17, 2006·Expert Opinion on Investigational Drugs·Frederick W K Tam
Mar 2, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Chihiro SuzukiEiji Kobayashi
Sep 8, 2006·Biochemical and Biophysical Research Communications·Chihiro SuzukiUichi Ikeda
Jun 18, 2016·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Agnes HacklLutz Thorsten Weber
Nov 28, 2017·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Agnes HacklLutz T Weber
May 12, 2016·Experimental and Therapeutic Medicine·Dechao XuXiang Gao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.