Prevention of hepatic ischemia/reperfusion injury by prolonged delivery of nitric oxide to the circulating blood in mice

Transplantation
Hidemasa KatsumiMitsuru Hashida

Abstract

To protect hepatocytes from ischemia/reperfusion injury in mice, prolonged delivery of nitric oxide (NO) to the liver was performed by a bolus intravenous injection of polyethylene glycol (PEG)-conjugated bovine serum albumin (BSA) with about 10 NO molecules attached via an S-nitrosothiol linkage (PEG-poly SNO-BSA). A hepatic ischemia/reperfusion injury was induced in mice by occluding the portal vein and the hepatic artery for 15 min followed by 6 hours of reperfusion. Each NO donor was injected into the tail vein just before the initiation of the reperfusion at a dose of 2 micromol NO/kg. The ischemia followed by reperfusion resulted in a striking increase in plasma alanine aminotransferase and aspartate aminotransferase activities. S-nitroso-N-acetyl penicillamine and NO-BSA, two classical S-nitrosothiols, had no statistically significant effect in preventing elevation of the markers. In marked contrast, PEG-poly SNO-BSA significantly (P<0.01) suppressed it. In addition, PEG-poly SNO-BSA significantly (P<0.01) reduced the number of neutrophils infiltrating into the liver and prevented the excessive production of NO from inducible NO synthase in the liver. These results indicate that PEG-poly SNO-BSA can be used to prevent he...Continue Reading

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Citations

Mar 28, 2008·Respiratory Research·Niels P van der KaaijAd J J C Bogers
Feb 8, 2011·Journal of Clinical Biochemistry and Nutrition·Ian N Hines, Matthew B Grisham
Dec 25, 2010·World Journal of Gastroenterology : WJG·Arunotai SiriussawakulJohn D Lang
Jul 8, 2011·World Journal of Gastroenterology : WJG·Hassen Ben AbdennebiJoan Roselló-Catafau
Sep 26, 2015·Pharmaceutics·Kazuaki TaguchiMasaki Otagiri
Feb 14, 2013·Nitric Oxide : Biology and Chemistry·Yu IshimaToru Maruyama
Aug 4, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hidemasa KatsumiMitsuru Hashida
Oct 23, 2008·Archives of Biochemistry and Biophysics·Yuta AbeMatthew B Grisham
Feb 10, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Mahmoud Abu-AmaraBarry Fuller
May 29, 2015·Acta Cirúrgica Brasileira·Márcio Luís LucasAngelo Alves de Mattos
Apr 26, 2017·Expert Review of Gastroenterology & Hepatology·Arash Nickkholgh, Daniel Maluf
Jan 4, 2019·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Eun Kyung ChoiDong Gun Lim
Dec 23, 2016·World Journal of Hepatology·Kyota Fukazawa, John D Lang

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