Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue

Scientific Reports
Justin Christopher D'AntinRalph Michael

Abstract

In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (H2O2) or distilled water (H2Od),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM H2O2 or H2Od or used as controls, and cultured for one month, during which dark field and tilt illumination photos were taken. These were used to observe and quantify, time until cellular growth and confluence on the posterior capsule. After culture, histological sections were stained for H&E, α-SMA, Ki-67 and vimentin and evaluated. We prevented cellular growth in 50% of H2Od and 58% H2O2 of treated samples. The overall prevention of cell growth compared to cultured controls was significant for both treatments while there was no significant difference between them. In the cases where cellular growth was not prevented, both treatments significantly delay cellular growth. Until day 28 none of the treated samples of either type that had shown growth reached total confluence. All cultured controls reached total confluence before treated samples (median = day 11.5). Also, histologically, there was a clear morphological difference betwe...Continue Reading

References

Sep 1, 1992·Survey of Ophthalmology·D J AppleA M Kostick
Dec 1, 1983·Ophthalmology·P J McDonnellW R Green
Jun 1, 1981·Acta Ophthalmologica·D S HullV Livingston
Aug 26, 1999·The British Journal of Ophthalmology·N OhguroY Tano
Jul 29, 2003·Korean Journal of Ophthalmology : KJO·Min-Ho KimJin-Seong Yoo
Jan 1, 1964·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·C D BINKHORST, M H GOBIN
May 8, 2004·Journal of Cataract and Refractive Surgery·Jonathan G CrowstonAnthony Maloof
Jun 24, 2004·Journal of Cataract and Refractive Surgery·James A Davison
Dec 25, 2004·Journal of Cataract and Refractive Surgery·Viviana FernandezJean-Marie Parel
May 26, 2007·Investigative Ophthalmology & Visual Science·George DuncanIan Michael Wormstone
Aug 19, 2007·Molecular Biology of the Cell·Frederique M D TholozanRoy A Quinlan
Nov 18, 2008·Experimental Eye Research·I Michael WormstoneChristopher S C Liu
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jun 15, 2010·American Journal of Ophthalmology·Gustavo A MontenegroRalph Michael
Jul 27, 2010·Journal of Cataract and Refractive Surgery·Georgia ClearyJohn Marshall
Nov 20, 2010·Investigative Ophthalmology & Visual Science·Qing PanMarjorie F Lou
Aug 13, 2011·Klinische Monatsblätter für Augenheilkunde·O K KlaprothT Kohnen
Feb 23, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Xiao HuangRui-Li Wei
Jan 28, 2014·Journal of Cataract and Refractive Surgery·David J SpaltonI Michael Wormstone
Feb 11, 2015·Journal of Cataract and Refractive Surgery·Jay S PeposeGregg Feinerman
Mar 19, 2015·Experimental Eye Research·Lisanne M NibourgSteven A Koopmans

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Citations

Dec 6, 2019·Eye·Andreea D Fișuș, Oliver Findl
May 3, 2019·Investigative Ophthalmology & Visual Science·Jacquelyn GerhartMindy George-Weinstein
Sep 26, 2020·Progress in Retinal and Eye Research·I M WormstoneJ A Eldred
Jul 3, 2021·Journal of Clinical Medicine·Joanna KonopińskaIwona Obuchowska

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