Prevention of renal damage caused by Shiga toxin type 2: Action of Miglustat on human endothelial and epithelial cells

Toxicon : Official Journal of the International Society on Toxinology
Magalí C GirardMaría M Amaral

Abstract

Typical hemolytic uremic syndrome (HUS) is responsible for acute and chronic renal failure in children younger than 5 years old in Argentina. Renal damages have been associated with Shiga toxin type 1 and/or 2 (Stx1, Stx2) produced by Escherichia coli O157:H7, although strains expressing Stx2 are highly prevalent in Argentina. Human glomerular endothelial cells (HGEC) and proximal tubule epithelial cells are very Stx-sensitive since they express high levels of Stx receptor (Gb3). Nowadays, there is no available therapy to protect patients from acute toxin-mediated cellular injury. New strategies have been developed based on the Gb3 biosynthesis inhibition through blocking the enzyme glucosylceramide (GL1) synthase. We assayed the action of a GL1 inhibitor (Miglustat: MG), on the prevention of the renal damage induced by Stx2. HGEC primary cultures and HK-2 cell line were pre-treated with MG and then incubated with Stx2. HK- 2 and HGEC express Gb3 and MG was able to decrease the levels of this receptor. As a consequence, both types of cells were protected from Stx2 cytotoxicity and morphology damage. MG was able to avoid Stx2 effects in human renal cells and could be a feasible strategy to protect kidney tissues from the cytotox...Continue Reading

References

Apr 1, 1996·Trends in Microbiology·C A Lingwood
Jan 4, 1998·Kidney International·H A Repetto
May 18, 1999·The Journal of Biological Chemistry·L LeeJ A Shayman
Jun 7, 2000·The Journal of Clinical Investigation·A AbeJ A Shayman
May 16, 2002·The Journal of Biological Chemistry·Thomas KolterKonrad Sandhoff
Jul 10, 2002·Current Opinion in Nephrology and Hypertension·Anna RichardsTimothy H J Goodship
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Apr 13, 2004·The Journal of Biological Chemistry·Marie E FraserMichael N G James
Mar 23, 2005·Lancet·Phillip I TarrWayne L Chandler
Oct 26, 2005·Microbes and Infection·Virginia Pistone CreydtCristina Ibarra
May 19, 2007·Molecular Genetics and Metabolism·Kerry Anne McEachernJohn Marshall
Jul 13, 2007·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A TreiberM Clozel
Sep 9, 2011·Cellular Microbiology·Vernon L Tesh
Jun 10, 2014·The Journal of Pathology·Stefan PorubskyChristoph Betz

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Citations

Jun 23, 2021·Pediatric Research·Daiana S SánchezClaudia Silberstein

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