Prevention of spontaneous abortion in the CBA x DBA/2 mouse model by intravaginal TGF-beta and local recruitment of CD4+8+ FOXP3+ cells

American Journal of Reproductive Immunology : AJRI
David A ClarkDaljeet Banwatt

Abstract

Activation of latent transforming growth factor (TGF)-beta in seminal plasma has been suggested by Robertson et al. to promote maternal tolerance to paternal antigens. A possible consequence reported by Tremellen et al. is increased pregnancy rates in women undergoing IVF. A decreased spontaneous abortion rate has also been postulated. Seminal plasma contains many factors besides TGF-beta, and a critical test of the hypothesis was required. The purpose of the present study was to directly test the effect of pure TGF-beta. Pharmaceutical grade bioactive TGF-beta3 with a bovine serum albumin (BSA) carrier 0.1-1% in phosphate-buffered saline (PBS) was given into the vaginal tract of CBA/J female mice at the time of mating with DBA/2 males. One microgram Salmonella enteritidis lipopolysaccharide was given intraperitoneally to augment occult losses and spontaneous resorptions assessed on day 13.5 of pregnancy. The effect of TGF-beta3 on recruitment of lymphomyeloid cells to the vaginal wall and vaginal lumen of unmated mice in estrus was assessed using immunohistochemistry and flow cytometry. Two nanogram of intravaginal TGF-beta3 in 0.1% BSA-PBS or 20 ng in 1% BSA-PBS reduced abortion rates. Protection was comparable to that achiev...Continue Reading

References

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Citations

Aug 26, 2010·Nature Reviews. Rheumatology·Clare TowerIan Bruce
Mar 13, 2009·Human Reproduction Update·Leigh R GuerinSarah A Robertson
Jan 20, 2016·Seminars in Immunopathology·David A Clark
Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·John J Bromfield
Jan 22, 2017·Cold Spring Harbor Perspectives in Biology·Shomyseh SanjabiMing O Li
Dec 23, 2017·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhi-Nang YinJing Yang
Dec 1, 2017·Reproductive Sciences·Bizhan Sadighi-MoghaddamSeyed Mohammad Moazzeni
Jan 30, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Elizabeth A Bonney, Stephen A Brown
Dec 31, 2009·American Journal of Reproductive Immunology : AJRI·David A ClarkRadslav Kinsky
Mar 25, 2010·American Journal of Reproductive Immunology : AJRI·Anne LeberAna C Zenclussen
Sep 8, 2011·Expert Review of Clinical Immunology·Stephy VargheseClare Tower
Mar 26, 2014·American Journal of Reproductive Immunology : AJRI·Anne Schumacher, Ana Claudia Zenclussen
Sep 16, 2014·Reproduction in Domestic Animals = Zuchthygiene·J E Schjenken, S A Robertson
Jan 31, 2020·Physiological Reviews·John E Schjenken, Sarah A Robertson
Feb 20, 2013·American Journal of Reproductive Immunology : AJRI·Ana Claudia Zenclussen
Jul 30, 2020·Reproductive Sciences·Hui ChengShuyun Zhao
Jan 27, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·David K Gardner
Jan 8, 2013·American Journal of Reproductive Immunology : AJRI·Ana TelesAnne Schumacher
Jul 15, 2015·Biology of Reproduction·John E SchjenkenSarah A Robertson
May 15, 2018·Frontiers in Endocrinology·Frank Nawroth, Michael von Wolff
Aug 9, 2011·American Journal of Reproductive Immunology : AJRI·Rosanna RamhorstGil Mor
Apr 13, 2011·American Journal of Reproductive Immunology : AJRI·Angela R Lemons, Rajesh K Naz
Jun 19, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·David J SharkeySarah A Robertson
Mar 14, 2013·American Journal of Reproductive Immunology : AJRI·Sarah A RobertsonLachlan M Moldenhauer
Sep 30, 2020·Journal of Reproductive Immunology·Beatriz GuerreroRaquel Tarazona
Jan 24, 2012·Cytokine·Stefano R GiannubiloMonica Emanuelli

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