PMID: 3141937Jul 1, 1988

Preventive effect of MCI-186 on 15-HPETE induced vascular endothelial cell injury in vitro

Prostaglandins, Leukotrienes, and Essential Fatty Acids
T WatanabeS Murota


Using cultured bovine aortic endothelial cells, the effects of MCI-186, a radical scavenger, were studied on arachidonic acid metabolism and on the cell injury caused by 15-HPETE. MCI-186 at 3 X 10(-5) M enhanced prostacyclin production in the intact endothelial cells without affecting phospholipase A2. When endothelial cell homogenates were used as an enzyme source, it was found that MCI-186 stimulated the conversion of arachidonic acid to prostacyclin like phenol, perhaps by trapping OH radicals produced in the process of the conversion of PGG2 to PGH2. On the other hand, MCI-186 was found to inhibit lipoxygenase metabolism of arachidonic acid in cell free homogenates of rat basophilic leukemia cells. The lipoxygenase inhibition caused by 3 X 10(-5) M MCI-186 was almost equivalent to that caused by 3 X 10(-6) M BW 755C. MCI-186 remarkably protected against endothelial cell damage caused by 15-HPETE. 3 X 10(-5) M of 15-HPETE caused endothelial cell death in about 60% of the population: however, pretreatment of the cells with 10(-5) M of MCI-186 or concomitant addition of 10(-5) M of MCI-186 with 15-HPETE to the cultures prevented the cell death completely. These results suggest that MCI-186 may become an unique anti-ischemic d...Continue Reading


Dec 11, 2007·Molecular Neurobiology·Venkata Prasuja NakkaRam Raghubir
Mar 23, 2004·European Journal of Pharmacology·Tsuyoshi TakemotoHiroshi Yamashita
Jun 1, 2005·European Journal of Pharmacology·Shimon AmemiyaYasuo Katayama
Feb 26, 2003·European Journal of Pharmacology·Osamu HoriikeHiroshi Yamashita
Sep 7, 2002·Life Sciences·Tai-Wing WuKwok-Pui Fung
Dec 7, 2002·Life Sciences·Kyuya Kogure
Nov 23, 2006·Journal of Neurotrauma·Kenji DohiTohru Aruga
May 31, 2003·Journal of Cardiovascular Pharmacology·Tetsuya HayashiYasushi Kitaura
Nov 24, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jiaoyun DongKen Ogo
Jan 1, 1990·Annals of the New York Academy of Sciences·S MurotaN Suda
Apr 29, 2008·Circulation Journal : Official Journal of the Japanese Circulation Society·Geethalakshmi RadhakrishnanShiro Sasaguri
Jun 14, 2002·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Masahiko Tanaka
Jul 26, 2005·Neurologia Medico-chirurgica·Masahiro MishinaYasuo Katayama
Jan 1, 2014·Future Neurology·Joseph S HudsonDavid M Hasan
Oct 5, 2011·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Syoichiro KonoKoji Abe
Jan 11, 2011·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Kenji IsahayaYasuhiro Hasegawa
Jul 27, 2010·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Yasumasa YamamotoRyo Oohara
Sep 8, 2007·Journal of Photochemistry and Photobiology. B, Biology·Mingzhang LinKeichi Nakagawa
May 30, 2007·The Journal of Thoracic and Cardiovascular Surgery·Yasuhiro KotaniShunji Sano


Nov 1, 1973·Journal of Neurochemistry·U PonténB K Siesjö

Related Concepts

Metabolic Process, Cellular
Phospholipase A2, human
Acute Basophilic Leukemia
Arachidonic Acid
Arachidonic Acid Metabolic Process
Cessation of Life
Bos taurus
MCI 186

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.