Abstract
The aim of this study was to investigate the effect of evodiamine (EV) on dexamethasone-induced osteoporosis in zebrafish. Zebrafish larvae were exposed to different concentrations of dexamethasone to obtain the osteoporosis in zebrafish. Calcium, phosphorus, and alizarin red staining determination were performed to evaluate the effects of EV on bone mineralization. Alkaline phosphatase (ALP), hydroxyproline (HP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits. The expression of MMP3-OPN-MAPK pathway in zebrafish was measured by Western blot. RT-PCR was used to determine mRNA levels of MMP3, OPN, and MAPK. EV could significantly increase the content of calcium and phosphorus. The results of alizarin red staining showed that EV could significantly increase the calcium sink of horse fish, increasing the area of bone formation. EV could increase the content of hydroxyproline in zebrafish. EV also increased ALP and TRAP in zebrafish. Western blot and RT-PCR results showed that EV restored the MMP3-OPN-MAPK pathway in zebrafish. In conclusion, we found that EV can alleviate dexamethasone-induced osteoporosis in zebrafish. The mechanism is related to activating MMP3-OPN-MAPK pathway and then acti...Continue Reading
References
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·W XuE E Medrano
Sep 8, 2004·Current Opinion in Pharmacology·Paul Goldsmith
Aug 24, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Jing WangJing-juan Pan
Mar 16, 2011·Journal of Cell Science·Julie C CrockettMiep H Helfrich
May 31, 2011·International Immunopharmacology·Eun Mi Choi
Oct 8, 2011·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Mei-Jie LiuDa-Hong Ju
Aug 20, 2014·Journal of Analytical Methods in Chemistry·Yuwei WangQiuju Li
Jan 22, 2015·International Journal of Experimental Pathology·Sara PasqualettiMassimo Mariotti
Apr 2, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Yuwei WangShuangrong Gao
Jan 26, 2017·Journal of Ethnopharmacology·Sharad DesaiArchana Paranjape
Mar 4, 2017·Journal of Ethnopharmacology·Yan YangRenhui Liu
Jun 1, 2017·Tissue Engineering. Part a·Alaa MansourFaleh Tamimi
Jun 7, 2017·Transplantation Proceedings·W Y ParkB H Chung
Nov 16, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Heng YinYong Ma
Citations
Oct 28, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Caiyan LiuHaiyang Yu
Jul 3, 2021·Pharmaceuticals·Siripat ChaichitMotoyuki Itoh
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Joana T RosaM Leonor Cancela
Aug 2, 2020·Journal of Ethnopharmacology·Qiang SunXiaofang Li