Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells

Stem Cell Research & Therapy
Yinshan BaiShouquan Zhang

Abstract

Male germline stem cells (mGSCs) offer great promise in regenerative medicine and animal breeding due to their capacity to maintain self-renewal and to transmit genetic information to the next generation following spermatogenesis. Human testis-derived embryonic stem cell-like cells have been shown to possess potential of mesenchymal progenitors, but there remains confusion about the characteristics and origin of porcine testis-derived stem cells. Porcine testis-derived stem cells were obtained from primary testicular cultures of 5-day old piglets, and selectively expanded using culture conditions for long-term culture and induction differentiation. The stem cell properties of porcine testis-derived stem cells were subsequently assessed by determining the expression of pluripotency-associated markers, alkaline phosphatase (AP) activity, and capacity for sperm and multilineage differentiation in vitro. The gene expression profile was compared via microarray analysis. We identified two different types of testis-derived stem cells (termed as C1 and C2 here) during porcine testicular cell culture. The gene expression microarray analysis showed that the transcriptome profile of C1 and C2 differed significantly from each other. The C1...Continue Reading

References

Feb 1, 1978·Biology of Reproduction·H W Van Straaten, C J Wensing
Oct 27, 1998·Tissue & Cell·M NaganoR L Brinster
Nov 20, 1998·Molecular Reproduction and Development·N H KimK S Chung
Dec 26, 2001·Biology of Reproduction·Koji YoshiokaShokichi Iwamura
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Dec 29, 2004·Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·F Kent HamraDavid L Garbers
Feb 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ren-Shan GeMatthew P Hardy
Mar 28, 2006·Nature·Kaomei GuanGerd Hasenfuss
Jul 28, 2006·Theriogenology·Jose Rafael Rodriguez-SosaAnn Hahnel
Mar 23, 2007·Biology of Reproduction·Sandeep GoelHiroshi Imai
Feb 19, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Bjoern PetersenHeiner Niemann
May 27, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Fariborz IzadyarFrancisco Silva
Aug 14, 2008·Molecular Human Reproduction·Athanasios ZovoilisWolfgang Engel
Oct 14, 2008·Nature·Sabine ConradThomas Skutella
Jun 2, 2009·Molecular and Cellular Endocrinology·Haolin ChenBarry R Zirkin
Jun 9, 2009·Journal of Molecular Cell Biology·Zhao WuLei Xiao
Oct 10, 2009·Human Reproduction·S C MizrakA M M van Pelt
Jan 20, 2010·Nature Reviews. Urology·Kehkooi KeePaul J Turek
Feb 12, 2010·Biology of Reproduction·Byung-Gak KimBuom-Yong Ryu
Jun 26, 2010·Nature·Kinarm KoHans R Schöler
Oct 12, 2010·Molecular Human Reproduction·Tatjana KhromovUlrich Zechner
Nov 12, 2013·Molecular Human Reproduction·J V ChikhovskayaA M M van Pelt
Nov 22, 2013·In Vitro Cellular & Developmental Biology. Animal·Madjid Momeni-MoghaddamAhmad Reza Bahrami
Dec 21, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Yi ZhengWenxian Zeng
May 24, 2014·Molecules and Cells·Na Young ChoiKinarm Ko
Jul 20, 2014·Stem Cells Translational Medicine·James F SmithNam D Tran
Jul 17, 2015·Reproduction, Fertility, and Development·Mahesh SahareHiroshi Imai
Jun 22, 2016·Cellular Reprogramming·Kyung Hoon LeeHyuk Song

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
light microscopy
flow cytometry
RNA-seq
ELISA
PCR

Software Mentioned

FlowJo
bowtie2
SPSS
tophat2

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.