PMID: 8604254Feb 1, 1996Paper

Prevotella intermedia fimbriae mediate hemagglutination

Oral Microbiology and Immunology
K P LeungW B Clark

Abstract

Our earlier studies demonstrated that clinical strains of Prevotella intermedia, isolated from human periodontal pockets, possess various types of fimbria (surface appendages) as determined ultrastructurally. These bacteria have the ability to agglutinate selected mammalian erythrocytes. Hemagglutinating activity exhibited by these cells may be attributable to these surface structures. Strain 17, which possess fimbriae of 8 nm in diameter and readily agglutinates human, monkey, sheep, rabbit, and mouse erythrocytes was selected to determine whether these fimbriae possessed the hemagglutinating activity. Fimbriae were mechanically sheared, concentrated by ammonium sulfate precipitation, solubilized in 10 mM Tris-HCl buffer containing 0.5% deoxycholate and partly purified by ultracentrifugation in a 10-50% linear sucrose gradient. Isolated fimbriae banded at a density of 1.20-1.15 g/ml, appeared fairly uniform ultrastructurally, and possessed hemagglutinating activity. The hemagglutinating activity of P. intermedia whole cells and isolated fimbriae was reduced by treatment with proteases and eliminated by treatment with heat at 80 degrees C for 10 min. The optimal pH for the hemagglutination was 7.0. In the process of hemagglutin...Continue Reading

References

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Citations

Feb 13, 2001·Journal of Clinical Periodontology·T BerglundhJ Lindhe
Jan 1, 1999·Oral Microbiology and Immunology·G Haraldsson, W P Holbrook
Aug 19, 2000·Infection and Immunity·K P Leung, B A Torres
Oct 16, 2008·BMC Microbiology·Maria D BoderoGeorge P Munson
Dec 19, 2009·Periodontology 2000·Atsuo Amano
Mar 27, 1999·Oral Microbiology and Immunology·S HamadaI Morisaki
Jun 9, 2004·Journal of Medical Microbiology·Nicola J Holden, David L Gally
Jun 17, 1998·FEMS Microbiology Letters·K P LeungC H Lai
Nov 24, 1998·Infection and Immunity·B R DornA Progulske-Fox

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