Prevotella intermedia induces matrix metalloproteinase-9 expression in human periodontal ligament cells

FEMS Microbiology Letters
Su-Min GuanJun-Zheng Wu

Abstract

Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured by semiquantitative reverse transcriptase PCR and its protein secretion was assayed by gelatin zymography. Prevotella intermedia ATCC 25611 supernatant time and dose-dependently induced MMP-9 expression. In contrast, Porphyromanas gingivalis ATCC 33277 supernatants, Escherichia coli lipopolysacchride and IL-1beta exhibited no stimulatory effects on MMP-9 production in hPDL cells. Mitogen-activated protein kinases [MAPK, including extracellular signal-related kinases (ERK), c-jun N-terminal kinases (JNK) and p38] inhibitors exerted no effect on the P. intermedia-induced MMP-9 production, indicating that P. intermedia induced MMP-9 production through an MAPK-independent pathway. Our results demonstrated that P. intermedia may contribute to periodontal tissue destruction during chronic periodontitis by inducing MMP-9 production in hPDL cells.

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Citations

Jan 19, 2016·Experimental and Molecular Pathology·Dimitry A ChistiakovYuri V Bobryshev
Apr 16, 2015·Mediators of Inflammation·Min WuShao-Yun Jiang
Aug 28, 2009·FEMS Microbiology Letters·Su-Min GuanJun-Zheng Wu
Nov 9, 2010·Journal of Dental Research·S-M GuanM Zhang
Oct 2, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·M Al-MemarD A MacIntyre
Feb 13, 2020·Critical Reviews in Microbiology·Niall A HickeyRebecca L Taylor

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