Prey capture and phagocytosis in the choanoflagellate Salpingoeca rosetta

PloS One
Mark J Dayel, Nicole King

Abstract

Choanoflagellates are unicellular and colonial aquatic microeukaryotes that capture bacteria using an apical flagellum surrounded by a feeding collar composed of actin-filled microvilli. Flow produced by the apical flagellum drives prey bacteria to the feeding collar for phagocytosis. We report here on the cell biology of prey capture in rosette-shaped colonies and unicellular "thecate" or substrate attached cells from the choanoflagellate S. rosetta. In thecate cells and rosette colonies, phagocytosis initially involves fusion of multiple microvilli, followed by remodeling of the collar membrane to engulf the prey, and transport of engulfed bacteria into the cell. Although both thecate cells and rosette colony cells produce ∼ 70 nm "collar links" that connect and potentially stabilize adjacent microvilli, only thecate cells were observed to produce a lamellipod-like "collar skirt" that encircles the base of the collar. This study offers insight into the process of prey ingestion by S. rosetta, and provides a context within which to consider potential ecological differences between solitary cells and colonies in choanoflagellates.

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Citations

Jan 20, 2015·Current Opinion in Microbiology·Nicholas A Lyons, Roberto Kolter
Jul 20, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stuart A Newman
Jun 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Arielle WoznicaNicole King
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Sep 22, 2017·Annual Review of Genetics·Frederique Varoqueaux, Dirk Fasshauer
May 24, 2018·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Charlene E LancasterMauricio R Terebiznik
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Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Lasse Tor NielsenAnders Andersen
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Apr 13, 2019·PLoS Biology·Davis LaundonPawel Burkhardt
Apr 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Carolyn L SmithRafael A Barrio
Dec 25, 2019·The Journal of Eukaryotic Microbiology·Nathan J L'Etoile, Christina King-Smith
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May 26, 2021·Current Biology : CB·Anne Houdusse, Margaret A Titus

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Methods Mentioned

BETA
light microscopy
electron microscopy

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