Abstract
The reaction center (RC) from the phototrophic bacterium Blastochloris viridis was the first integral membrane protein complex to have its structure determined by X-ray crystallography and has been studied extensively since then. It is composed of four protein subunits, H, M, L, and C, as well as cofactors, including bacteriopheophytin (BPh), bacteriochlorophyll (BCh), menaquinone, ubiquinone, heme, carotenoid, and Fe. In this study, we utilized mass spectrometry-based proteomics to study this protein complex via bottom-up sequencing, intact protein mass analysis, and native MS ligand-binding analysis. Its primary structure shows a series of mutations, including an unusual alteration and extension on the C-terminus of the M-subunit. In terms of quaternary structure, proteins such as this containing many cofactors serve to test the ability to introduce native-state protein assemblies into the gas phase because the cofactors will not be retained if the quaternary structure is seriously perturbed. Furthermore, this specific RC, under native MS, exhibits a strong ability not only to bind the special pair but also to preserve the two peripheral BCh's.
References
Jan 11, 1978·Biochimica Et Biophysica Acta·D HoltenJ P Thornber
Jun 4, 1991·Biochemistry·O el-KabbaniM Schiffer
Dec 1, 1990·Protein Engineering·G von Heijne, C Manoil
Feb 20, 1996·Biochemistry·J O GoldsmithS G Boxer
Sep 2, 1999·Biochemistry·C KirmaierD Holten
Dec 5, 2006·Nucleic Acids Research·Helen BermanJohn L Markley
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Sep 4, 2008·Proteomics·Sandra TanMaxey C M Chung
Nov 1, 2008·Nature Methods·Albert J R Heck
Jun 23, 2009·Biochimica Et Biophysica Acta·Nina S PonomarenkoJames R Norris
Jan 23, 2010·Molecular & Cellular Proteomics : MCP·Christopher M RyanJulian P Whitelegge
May 10, 2011·Annual Review of Biochemistry·Nelson P Barrera, Carol V Robinson
Nov 8, 2011·The Biochemical Journal·Aleksander W RoszakRichard J Cogdell
Jan 31, 2012·Nature Methods·Linda C JohanssonRichard Neutze
Mar 14, 2012·Analytical Chemistry·Nina Morgner, Carol V Robinson
Sep 21, 2012·Nature·Zachary D BlountRichard E Lenski
Mar 9, 2013·Nature Protocols·Arthur LaganowskyCarol V Robinson
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yufen CheSheng Luan
Dec 20, 2013·Nature Communications·Linda C JohanssonRichard Neutze
Aug 12, 2014·Nature Methods·David ArnlundRichard Neutze
Jan 17, 2015·Annual Review of Physical Chemistry·Shahid MehmoodCarol V Robinson
May 15, 2015·Nucleic Acids Research·Konstantinos D TsirigosArne Elofsson
Sep 10, 2015·Biological Chemistry·Albert KonijnenbergFrank Sobott
Nov 18, 2015·Biochemistry·Yue LuRobert E Blankenship
Jan 23, 2016·Genome Announcements·Lu-Ning LiuNeil Hall
Mar 26, 2016·Journal of the American Chemical Society·Xiao CongArthur Laganowsky
May 25, 2016·The Journal of Physical Chemistry. B·Nikki Cecil M MagdaongRobert E Blankenship
Aug 11, 2016·Journal of the American Society for Mass Spectrometry·Hao ZhangMichael L Gross
Sep 10, 2016·Molecular Membrane Biology·Shaun RawsonStephen P Muench
Dec 3, 2016·Journal of the American Society for Mass Spectrometry·Aneika C Leney, Albert J R Heck
Mar 2, 2017·Acta Crystallographica. Section A, Foundations and Advances·Amit SharmaGergely Katona
Jul 25, 2017·Expert Review of Proteomics·Upendra K KarJulian P Whitelegge