PMID: 6170936Sep 11, 1981Paper

Primary and secondary structures of Escherichia coli MRE 600 23S ribosomal RNA. Comparison with models of secondary structure for maize chloroplast 23S rRNA and for large portions of mouse and human 16S mitochondrial rRNAs

Nucleic Acids Research
C BranlantH Kössel

Abstract

We determined 90% of the primary structure of E.coli MRE 600 23S rRNA by applying the sequencing gel technique to products of T1, S1, A and Naja oxiana nuclease digestion. Eight cistron heterogeneities were detected, as well as 16 differences with the published sequence of a 23S rRNA gene of an E.coli K12 strain. The positions of 13 post-transcriptionally modified nucleotides and of single-stranded, double-stranded and subunit surface regions of E.coli 23S rRNA were identified. Using these experimental results and by comparing the sequences of E.coli 23S rRNA, maize chloro. 23S rRNA and mouse and human mit 16S rRNAs, we built models of secondary structure for the two 23S rRNAs and for large portions of the two mit rRNAs. The structures proposed for maize chloroplast and E.coli 23S rRNAs are very similar, consisting of 7 domains closed by long-range base-pairings. In the mitochondrial 16S rRNAs, 3 of these domains are strongly reduced in size and have a very different primary structure compared to those of the 23S rRNAs. These domains were previously found to constitute a compact area in the E.coli 50S subunits. The conserved domains do not belong to this area and contain almost all the modified nucleotides. The most highly cons...Continue Reading

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E A MorganM Nomura
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D A Peattie
Nov 1, 1979·FEBS Letters·C BranlantJ P Ebel
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·F Dohme, K H Nierhaus
Aug 7, 1975·Nature·G E Fox, C R Woese
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J M Pipas, J E McMahon
Dec 31, 1973·Molecular & General Genetics : MGG·C A MorrisonG Stöffler
Dec 2, 1974·European Journal of Biochemistry·P GreenwellR H Symons
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J BrosiusH F Noller
Jul 31, 1980·Nature·I C EperonD P Nierlich
Oct 6, 1980·FEBS Letters·R N Nazar
Feb 25, 1981·Nucleic Acids Research·C BranlantM Jacob
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H NomiyamaY Takagi
Feb 14, 1980·Nature·M A Wild, R Sommer

❮ Previous
Next ❯

Citations

Jan 1, 1987·Journal of Molecular Evolution·J L GorskiR D Schmickel
Sep 20, 1991·Journal of Molecular Biology·A V MunishkinA B Chetverin
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Jan 1, 1986·Progress in Biophysics and Molecular Biology·K Nagano, M Harel
Jan 1, 1988·Progress in Biophysics and Molecular Biology·H A RauéW Musters
Jan 1, 1986·Bio Systems·S A Gerbi
Jul 20, 2002·Current Opinion in Structural Biology·Robin R GutellJamie J Cannone
Feb 14, 2012·ACS Chemical Biology·Yogo Sakakibara, Christine S Chow
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A BartaE Kuechler
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G T OostergetelM Boublik
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I L GonzalezR D Schmickel
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V F de la CruzL Simpson
Aug 11, 1982·Nucleic Acids Research·H G Köchel, H Küntzel
Dec 20, 1982·Nucleic Acids Research·G LapalmeD Sankoff
Jul 25, 1983·Nucleic Acids Research·D R HagueP D Collins
Jan 25, 1983·Nucleic Acids Research·F Sor, H Fukuhara
Nov 25, 1983·Nucleic Acids Research·V C WareS A Gerbi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.