PMID: 8941979Sep 1, 1996Paper

Primary bovine hepatocytes in the study of cytokine induced acute-phase protein secretion in vitro

Veterinary Immunology and Immunopathology
S P AlsemgeestE Gruys

Abstract

To investigate the utility of primary cultures of bovine hepatocytes for compartmentalized acute phase protein studies the secretion of serum amyloid-A (SAA) and haptoglobin (Hp) was measured after stimulation by pro-inflammatory cytokines (recombinant human IL-6 (rhIL-6) and recombinant human tumour necrosis factor-alpha (rhTNF-alpha)). During the incubation period of the experiment, the SAA and Hp secretion into the culture medium increased (P < 0.05). SAA concentrations showed an additional increase following treatment with each of the cytokines (P < 0.01). Hp concentrations remained unchanged, whereas incubation with a combination of both resulted in a significant increase of the medium concentration of both SAA (P < 0.01) and Hp (P < 0.05). From these findings it is concluded that primary bovine hepatocytes can be used for in vitro studies on acute-phase protein secretion.

References

May 1, 1992·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G A van 't KloosterA S van Miert
Dec 1, 1992·Journal of Veterinary Pharmacology and Therapeutics·A S van MiertT Wensing
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Citations

May 8, 2008·Veterinary Research Communications·S R LomborgS Jacobsen
Aug 25, 2004·Comparative Immunology, Microbiology and Infectious Diseases·T OrroT Soveri
May 26, 2004·The Veterinary Journal·H MurataM Yoshioka
Oct 26, 2013·The Veterinary Record·K CrawfordA G Gow
Aug 22, 2000·The Veterinary Quarterly·H J KarremanT Wensing
Apr 7, 2007·The Veterinary Journal·Toomas OrroTimo Soveri
Jul 29, 2015·Veterinary Parasitology·Brian LassenToomas Orro
Apr 19, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Hyeok-Joong KangMyunggi Baik
May 27, 1999·The Veterinary Record·N U HoradagodaP D Eckersall
Aug 22, 2008·Journal of Veterinary Science·Eun Joo KimKyoung Ho Choi
Nov 2, 2018·BMC Veterinary Research·Paulina JaworTadeusz Stefaniak

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