PMID: 9523701Apr 2, 1998Paper

Primary cause of mortality in the armyworm larvae simultaneously parasitized by parasitic wasp and infected with bacteria

European Journal of Biochemistry
H MatsumotoY Hayakawa

Abstract

Parasitoid wasps never kill their hosts before the wasp larvae emerge from the host. However, almost 100% of the host armyworm larvae Pseudaletia separata die within 2-3 days by parasitization with the wasp Cotesia kariyai or by injection of polydnavirus, the wasp symbiont virus, when they are simultaneously infected by the pathogenic bacterium Serratia marcescens. The present study was conducted to elucidate the crucial factor causing this larval mortality. An insecticidal protein has been shown to exist in the hemolymph of dying host larvae; it has been purified by procedures consisting of reverse-phase column extraction, gel filtration and ion-exchange column chromatography. The purified protein showed a strong insecticidal effect with a median lethal dosage (LD50) of 13 pmol/larva and was estimated to have a molecular mass of 57 kDa. The amino acid sequence of the insecticidal protein was partially characterized and used for isolation and sequencing of the genomic DNA. The deduced amino acid sequence for this protein revealed striking similarity with the metalloprotease of S. marcescens enterobacter.

Citations

Aug 6, 2003·European Journal of Biochemistry·Hitoshi MatsumotoYoichi Hayakawa
Dec 20, 2013·Biology Letters·Hideki NishikawaKikuo Iwabuchi
Oct 18, 2012·Research in Microbiology·Robert M Q ShanksJames E Fender
Apr 4, 2006·Journal of Insect Physiology·Angelika Fath-GoodinBruce A Webb
Apr 13, 2007·Archives of Insect Biochemistry and Physiology·F García-Gil De MuñozF C Hernández-Hernández

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.