Primary cell cultures from anaplastic thyroid cancer obtained by fine-needle aspiration used for chemosensitivity tests

Clinical Endocrinology
Alessandro AntonelliPaolo Miccoli

Abstract

Anaplastic thyroid cancer (ATC) is often inoperable and chemotherapy and radiotherapy are the main treatments. Until now, 'primary ATC cell cultures' (ANA) have been developed from surgical biopsies. We investigated the possibility of obtaining ANA from fine-needle aspiration (FNA-ANA) and testing their sensitivity to chemotherapeutic agents, which could enable treatments to be more effective and avoid unnecessary surgical procedures. The aim of this study was to obtain FNA-ANA from three ATC patients and to evaluate the chemosensitivity of FNA-ANA to chemotherapeutic agents. FNA-ANA from ATC patients were cultured in RPMI 1640 and propagated in Dulbecco's modified Eagle's medium (DMEM). Chemosensitivity was evaluated by inhibiting the proliferation (analysing the number of viable cells by the cleavage of tetrazolium salts), by increasing the concentration of four different chemotherapeutic agents: bleomycin, cisplatin, gemcitabine and etoposide. The chemotherapeutic agents significantly inhibited (> 50%) FNA-ANA proliferation. Another ANA for each patient was obtained from a surgical biopsy specimen; the results for the chemosensitivity tests were similar to those obtained using FNA-ANA. Our study demonstrates the possibility ...Continue Reading

References

Mar 1, 1991·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J L AbbruzzeseM N Raber
Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·W SchroyensJ Klastersky
Jan 1, 1985·Mayo Clinic Proceedings·C J NelC S Grant
Jan 1, 1995·Head & Neck·R K TanD P Shedd
Dec 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M J MillwardM Proctor
Jan 1, 1996·Head & Neck·T KobayashiM Monden
Nov 4, 2000·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D Giuffrida, H Gharib
Feb 24, 2001·Journal of Endocrinological Investigation·B BusnardoM E Girelli
Jun 30, 2001·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·R H CobinUNKNOWN Thyroid Carcinoma Task Force
Apr 27, 2004·Surgery Today·Minoru KiharaHiroyasu Yokomise
Nov 3, 2004·International Journal of Radiation Oncology, Biology, Physics·Renaud De CrevoisierMartin Schlumberger
Feb 3, 2005·American Journal of Clinical Oncology·Robert HaddadChristopher Sullivan
Mar 2, 2005·Cancer·Electron KebebewAlex McMillan
Sep 2, 2005·Pharmacology & Therapeutics·Paul Bentley, Pankaj Sharma
Oct 12, 2005·Biotechnology Annual Review·Michael V BerridgeAn S Tan
Oct 17, 2006·The Journal of Laryngology and Otology·E AbelardoP Tierney
Nov 30, 2006·Langenbeck's Archives of Surgery·Paolo MiccoliPiero Berti

❮ Previous
Next ❯

Citations

Jan 31, 2012·Current Oncology Reports·Alessandro AntonelliClodoveo Ferri
Jul 3, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Poupak FallahiAlessandro Antonelli
Aug 12, 2008·Thyroid : Official Journal of the American Thyroid Association·Giuliana SalvatoreMassimo Santoro
Jul 16, 2010·Journal of Oncology·Alessandro AntonelliPoupak Fallahi
Mar 20, 2015·International Journal of Molecular Sciences·Poupak FallahiAlessandro Antonelli
Jul 15, 2015·Expert Review of Anticancer Therapy·Silvia Martina FerrariPoupak Fallahi
Aug 30, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Alessandro AntonelliPaolo Miccoli
Dec 5, 2015·Frontiers in Endocrinology·Silvia Martina FerrariAlessandro Antonelli
Oct 19, 2016·Expert Review of Anticancer Therapy·Poupak FallahiAlessandro Antonelli
Feb 10, 2012·Yonsei Medical Journal·Sun Min LimByoung Chul Cho
Sep 19, 2009·Expert Review of Anticancer Therapy·Paolo MiccoliMichele N Minuto
Oct 11, 2017·Current Medicinal Chemistry·Silvia Martina FerrariPoupak Fallahi
Sep 11, 2019·International Journal of Molecular Sciences·Silvia Martina FerrariAlessandro Antonelli
Jan 22, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Leonardo CristinzianoMaria Rosaria Galdiero
Feb 8, 2011·The Journal of Clinical Endocrinology and Metabolism·Salvatore Benvenga
Jan 16, 2014·The Journal of Clinical Endocrinology and Metabolism·Alessandro AntonelliPaolo Miccoli
Jan 27, 2012·The Journal of Clinical Endocrinology and Metabolism·Alessandro AntonelliPaolo Miccoli
Nov 16, 2013·Experimental & Molecular Medicine·Brian T D TobeEvan Y Snyder
Jul 31, 2018·Expert Review of Endocrinology & Metabolism·Poupak FallahiSilvia Martina Ferrari
Mar 24, 2018·Molecular Medicine Reports·Poupak FallahiSilvia Martina Ferrari
Mar 23, 2011·Expert Review of Anticancer Therapy·Stefan L KojicSam M Wiseman
Jun 23, 2020·Seminars in Cancer Biology·Poupak FallahiAlessandro Antonelli
Feb 16, 2020·Best Practice & Research. Clinical Endocrinology & Metabolism·Alessandro AntonelliSilvia Martina Ferrari
Feb 29, 2020·Best Practice & Research. Clinical Endocrinology & Metabolism·Alessandro AntonelliPoupak Fallahi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.