Primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses

Avian Pathology : Journal of the W.V.P.A
K M ZaffutoC L Afonso

Abstract

A major route of infection of avian influenza virus (AIV) and Newcastle disease virus (NDV) in chickens is through cells of the respiratory epithelium. Here we describe the development of a method for culture of tracheal epithelial cells from chicken embryos as well as their use in studies of infection with avian respiratory viruses such as low-pathogenicity AIV and lentogenic NDV. Positive immunostaining for cytokeratin, the presence of cilia and microvilli, and microarray analysis of transcribed RNA demonstrated that the isolated cells were epithelial in nature. Infection of the epithelial cell cultures with AIV and NDV was demonstrated using immunofluorescence or green fluorescence protein fluorescence microscopy, respectively. Growth curves of AIV and NDV in tracheal epithelial cells revealed that tracheal epithelial cells can fully support AIV and NDV growth and reinfection. This system, which mimics that of the natural infection, will be useful to study the mechanisms of early viral infection and cellular host transcriptional responses.

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Citations

Sep 27, 2012·Cytotechnology·Shahla ShahsavandiNima Zarrin Lebas
Nov 28, 2012·PloS One·Noorjahan Banu AlitheenPeter McCullagh
Jun 24, 2011·Veterinary Immunology and Immunopathology·Fabienne RauwBénédicte Lambrecht
Jul 12, 2008·Veterinary Immunology and Immunopathology·Luciana SarmentoMary Pantin-Jackwood
Mar 13, 2014·Avian Pathology : Journal of the W.V.P.A·José Francisco Rivera-BenitezHumberto Ramírez-Mendoza
Jul 28, 2016·Viruses·Neda BarjestehShayan Sharif
Jun 30, 2019·Avian Diseases·Mohammad F HossainPriscilla F Gerber

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Datasets Mentioned

BETA
D00844

Methods Mentioned

BETA
PCR
light microscopy
transmission electron microscopy
electrophoresis

Software Mentioned

2U
GeneSpring GX
GenePix Pro
GenePix
ACT

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