Primary culture and plasmid electroporation of the murine organ of Corti.

Journal of Visualized Experiments : JoVE
Mark ParkerAlbert S B Edge

Abstract

In all mammals, the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea of the inner ear (fig 1). Hair cells in the developing cochlea, which are the mechanosensory cells of the auditory system, are aligned in one row of inner hair cells and three (in the base and mid-turns) to four (in the apical turn) rows of outer hair cells that span the length of the organ of Corti. Hair cells transduce sound-induced mechanical vibrations of the basilar membrane into neural impulses that the brain can interpret. Most cases of sensorineural hearing loss are caused by death or dysfunction of cochlear hair cells. An increasingly essential tool in auditory research is the isolation and in vitro culture of the organ explant. Once isolated, the explants may be utilized in several ways to provide information regarding normative, anomalous, or therapeutic physiology. Gene expression, stereocilia motility, cell and molecular biology, as well as biological approaches for hair cell regeneration are examples of experimental applications of organ of Corti explants. This protocol describes a method for the isolation and culture of the organ of Corti from neonatal mice. The accompanyi...Continue Reading

References

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Jan 13, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer M JonesMatthew W Kelley
Jan 13, 2006·Journal of Neurobiology·Rodrigo Martinez-MonederoAlbert S B Edge
Feb 20, 2009·The Journal of Comparative Neurology·Daniela Buckiová, Josef Syka

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Citations

Apr 8, 2011·Journal of the Association for Research in Otolaryngology : JARO·Mark A ParkerAlbert S B Edge
Apr 25, 2014·Science Translational Medicine·Jeremy L PinyonGary D Housley
May 3, 2011·Hearing Research·Abraham M SheffieldRichard J H Smith
Apr 29, 2016·BMC Genomics·Sabrina RiccardiBernd Kinzel
Mar 19, 2016·Scientific Reports·Judith S KempfleAlbert S B Edge
Aug 5, 2016·The Journal of Experimental Biology·Pei-Ciao TangGlen M Watson
May 28, 2019·Frontiers in Cellular Neuroscience·Jacqueline M OgierBryony A Nayagam
Mar 5, 2013·Bio-protocol·Omar Akil, Lawrence R Lustig
Dec 30, 2020·JCI Insight·Katharine A FernandezAlbert Sb Edge
Feb 5, 2021·Scientific Reports·Jerome NevouxAlbert S B Edge
May 23, 2020·Stem Cell Reports·Pei-Ciao TangRick F Nelson

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Methods Mentioned

BETA
dissection
transgenic

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