PMID: 8946692Sep 1, 1996Paper

Primary culture of Borrelia burgdorferi from Ixodes ricinus ticks

Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology
M M WittenbrinkH Krauss

Abstract

A total of 971 Ixodes (I.) ricinus ticks field-collected in May and June 1993 and 1994 in two different geographic regions (Middle Hesse and Lower Saxony) were examined for the presence of Lyme borreliosis spirochetes (Borrelia burgdorferi sensu lato) by primary culture using nonselective and selective BSK II medium. Modifications of BSK II medium with varying concentrations of bovine serum albumin and with rabbit serum being replaced by horse serum were examined for their ability to support growth of borreliae from ticks. Spirochetes were isolated from 17.8% each of female and male I. ricinus from Lower Saxony. Isolation rates from female and male I. ricinus originating from Middle Hesse were 13.5% and 19.6%, respectively. In comparison to nonselective BSK II medium the isolation rate of borreliae from the tick midgut was increased significantly (35%) by additional culture in selective BSK II medium. Most spirochetal isolates (85%) were detected within three weeks of incubation. Compared to isolation in standard BSK II medium, reduction of bovine serum albumin by 50% did not diminish the isolation rate of borreliae from ticks. Spirochetal isolates were identified as Borrelia burgdorferi sensu lato by PCR using a primer set bas...Continue Reading

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Citations

Nov 5, 2005·Applied and Environmental Microbiology·Carolin Rauter, Thomas Hartung
Aug 7, 2001·The Veterinary Record·S SpeckM M Wittenbrink
Aug 17, 1999·Journal of Clinical Microbiology·D PosticG Baranton

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