PMID: 7129482Aug 1, 1982

Primary cultures and the levels of cytochrome P450 in hepatocytes from mouse, rat, hamster, and rabbit liver

In Vitro
C J Maslansky, G M Williams

Abstract

Hepatocyte primary cultures (HPC) derived from rat, mouse, hamster, and rabbit liver were characterized for a variety of parameters. The conditions that maximized recovery, attachment, and survival varied between species. Hepatocytes from all four species were capable of attaching in serum-free Williams' medium E (WME), but optimal attachment as monolayer cultures was achieved for mouse and hamster HPC in medium receiving 1% calf serum supplementation. Hamster hepatocytes required additional cations, whereas rabbit and rat hepatocytes displayed maximal attachment in medium supplemented with 10% calf serum. Survival of mouse and rabbit hepatocytes after 24 h in serum supplemented media was in the order of 90%. Rat and hamster hepatocyte 24 h survival was approximately 70 and 60%, respectively, and was not significantly affected by serum supplementation. Hepatocytes from each species varied in their content of cytochrome P450 at the time of isolation and in the rate of reduction during culture. Mouse and rat hepatocytes demonstrated the most rapid decline in content during the initial 24 h in culture, whereas concentrations in rabbit hepatocytes were virtually unchanged. The rate of decline in P450 concentrations in hamster hepat...Continue Reading

References

Jan 1, 1979·Archives of Biochemistry and Biophysics·W E FahlH C Pitot
Feb 1, 1979·Archives of Biochemistry and Biophysics·D M Bissell, P S Guzelian
Nov 1, 1975·The Biochemical Journal·I J Arinze, D L Rowley
Jan 1, 1976·Methods in Cell Biology·P O Seglen
Feb 1, 1978·Environmental Health Perspectives·R T Williams
Jul 1, 1979·Experimental Cell Research·R C HughesR R Dourmashkin
Sep 12, 1979·Biochemical and Biophysical Research Communications·B J Blaauboer, A J Paine
Dec 7, 1977·Biochemical and Biophysical Research Communications·M HöökA Vaheri
Oct 1, 1977·Archives of Biochemistry and Biophysics·J Zaleski, J Bryla
Jan 1, 1976·Methods in Cell Biology·D R LaBrecque, R B Howard
Mar 1, 1972·European Journal of Clinical Pharmacology·B SchoeneH F von Oldershausen
Nov 1, 1974·Experimental Cell Research·G M Williams, J M Gunn
Oct 1, 1981·In Vitro·J E KlaunigB F Trump
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A McQueenG M Williams
Apr 1, 1981·Toxicology and Applied Pharmacology·C A McQueenG M Williams
Oct 1, 1981·In Vitro·J E KlaunigB F Trump
Nov 1, 1947·The American Journal of Anatomy·S H WEBSTERD J ZIMMER

Citations

Mar 1, 1993·Journal of Applied Toxicology : JAT·Y OshiroE Rohrbacher
Jun 1, 1987·Cell Biology and Toxicology·T BarfknechtD Kornbrust
Sep 1, 1987·Cell Biology and Toxicology·R LangenbachS Nesnow
Jun 1, 1986·Cell Biology and Toxicology·C A McQueenG M Williams
Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G K InnesK E Hobbs
Apr 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y VandenbergheA Guillouzo
Sep 14, 2001·European Journal of Drug Metabolism and Pharmacokinetics·G UbeaudP Coassolo
Feb 1, 1985·Mechanisms of Ageing and Development·C J Maslansky, G M Williams
Jan 1, 1991·Mutation Research·B G BoyesR Stapley
May 1, 1993·Toxicology Letters·N J DelRaso
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A GuillouzoC Guguen-Guillouzo
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J N Lawrence, D J Benford
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W C MennesB J Blaauboer
Feb 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K MüllerL Müller
Apr 18, 2003·Biomaterials·Jieliang LiYaoting Yu
Jul 12, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C EeckhoutteM Dacasto
Feb 6, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A J Paine, Evangelos Andreakos
Oct 15, 1996·Environmental Toxicology and Pharmacology·A J Paine
Nov 14, 2000·Tissue Engineering·K BehniaM Toner
May 26, 1998·Environmental Health Perspectives·A Guillouzo
Apr 1, 1984·Food Additives and Contaminants·G M Williams
Jan 1, 1994·In Vitro Cellular & Developmental Biology. Animal·H O JaureguiC Mullon
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·R A Smith
Jan 23, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Myung Hee KimNam-Joon Cho
Apr 6, 2005·The Journal of Cell Biology·Catherine GondcailleMaurice Bugaut
Jun 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·C Guguen-GuillouzoA Guillouzo
Aug 1, 1985·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M WarrenM Balls
Jun 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Y Nishibe, M Hirata
Sep 1, 1991·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H O JaureguiD J Waxman
May 1, 1992·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G A van 't KloosterA S van Miert
Oct 15, 1984·FEBS Letters·A P Wolffe, J R Tata
Oct 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Adrian Reuben
Nov 14, 2013·Biotechnology and Bioengineering·Lorenna D BuckLinda G Griffith
Jun 20, 2014·International Journal of Experimental Pathology·Satoshi MiyaharaShin-ichi Yoshida
Jun 1, 1985·Regulatory Toxicology and Pharmacology : RTP·G M Williams
Mar 1, 1984·Cancer Letters·A L HellemannG M Williams
Jan 1, 1983·Environmental Mutagenesis·C A McQueenG M Williams
Jan 1, 1984·Environmental Mutagenesis·D Kornbrust, T Barfknecht
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J M BegueA Guillouzo
Jan 1, 1983·Annals of the New York Academy of Sciences·A Dipple, C A Bigger
Jan 1, 1986·Environmental Mutagenesis·M J Miller, C A McQueen
Dec 1, 1987·Journal of Applied Toxicology : JAT·Y OshiroC E Piper
May 15, 1986·Biochemical Pharmacology·S AhmadD T Vistica
Mar 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H E WalpoleJ W Kennedy
Jan 1, 1984·Toxicologic Pathology·J E KlaunigB F Trump
Jan 1, 1983·Annals of the New York Academy of Sciences·C A McQueen, G M Williams
Apr 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J E KlaunigP J Goldblatt
Jan 1, 1986·Teratogenesis, Carcinogenesis, and Mutagenesis·L A OglesbyR J Kavlock
Jan 13, 2006·The Journal of Biological Chemistry·Yasuhiro Ishihara, Norio Shimamoto
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F BalletR Infante
Dec 10, 1998·Journal of Biomedical Science·H L ChenD S Chen

Related Concepts

Metazoa
Cell Adhesion
Cell Survival
Cytochrome P-450 Oxygenase
Cricetus
Liver
Mesocricetus
Chinchilla Rabbits
Rats, Inbred F344
Mouse, Swiss

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.