PMID: 7028109Sep 29, 1981Paper

Primary deuterium and tritium isotope effects upon V/K in the liver alcohol dehydrogenase reaction with ethanol

Biochemistry
S E Damgaard

Abstract

The primary isotope effect upon V/K when ethanol stereospecifically labeled with deuterium or tritium is oxidized by liver alcohol dehydrogenase has been measured between pH 6 and 9. The deuterium isotope effect was obtained with high reproducibility by the use of two different radioactive tracers, viz. 14C and 3H, to follow the rate of acetaldehyde formation from deuterium-labeled ethanol and normal ethanol, respectively. Synthesis of the necessary labeled compounds is described in this and earlier work referred to. V/K isotope effects for both tritium and deuterium have been measured with three different coenzymes, NAD+, thio-NAD+, and acetyl-NAD+. With NAD+ at pH 7, D(V/K) was 3.0 and T(V/K) was 6.5. With increasing pH, these values decreased to 1.5 and 2.5 at pH 9. The intrinsic isotope effect evaluated by the method of Northrop [Northrop, D.B. (1977) in Isotope Effects on Enzyme-Catalyzed Reactions (Cleland, W. W., O'Leary, M, H., & Northrop, D. B., Eds.) pp 112-152, University Park Press, Baltimore] varies little with pH. It amounts to about 10 with NAD+ and about 5 with the coenzyme analogues. Commitment functions and their dependence upon pH calculated in this connection appear to be in agreement with known kinetic para...Continue Reading

References

Oct 1, 1977·The Biochemical Journal·S E Damgaard
Jan 1, 1976·Frontiers of Gastrointestinal Research·G Ugarte, H Iturriaga
Mar 25, 1975·Biochemistry·J T McFarland, Y H Chu
Nov 24, 1970·Biochemistry·J D Shore, H Gutfreund
Nov 25, 1970·Biochemical and Biophysical Research Communications·N Grunnet
Dec 1, 1971·Archives of Biochemistry and Biophysics·J D Shore, R L Brooks
Dec 1, 1971·The Biochemical Journal·M J ArslanianJ G Reinhold
Dec 1, 1972·Canadian Journal of Biochemistry·C S HanesJ T Wong
Feb 1, 1973·Archives of Biochemistry and Biophysics·H Gershman, R H Abeles
Jan 1, 1973·Analytical Biochemistry·L E Anderson, W O McClure
Jan 1, 1970·Acta Chemica Scandinavica·O MarkovicS Rao
Jun 1, 1966·Biochemistry·A H Blair, B L Vallee
Jun 1, 1959·Archives of Biochemistry and Biophysics·J M SIEGELR M BOCK
Sep 1, 1963·Biochemistry·A M STEINB M ANDERSON

❮ Previous
Next ❯

Citations

Mar 24, 2007·Rejuvenation Research·Mikhail S Shchepinov
Oct 11, 2012·PloS One·Zizhong LiNora D Volkow
Jul 1, 1989·Pharmacology & Toxicology·F Lundquist, L L Hansen
Nov 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G T Miwa, A Y Lu
Oct 1, 1988·Alcoholism, Clinical and Experimental Research·T CronholmS Skagerberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.