Abstract
N-Formyl-methionyl-leucyl-phenylalanine (fMLP) is a potent activator of neutrophil degranulation. The intracellular signaling mechanisms involved in the potentiating effect of fibrinogen on fMLP-induced primary granule release from human neutrophils were investigated. Fibrinogen caused a significant leftward shift of the concentration-response curve of fMLP-induced elastase release. An antibody against Mac-1 (CD11b/CD18) prevented the potentiating effect of fibrinogen, suggesting that soluble fibrinogen potentiates fMLP-induced degranulating effect by a mechanism mediated by the integrin Mac-1. Fibrinogen enhanced fMLP-induced tyrosine phosphorylation in human neutrophils and markedly enhanced the phosphorylation of mitogen-activated protein kinases (MAPK) caused by fMLP. However, U0126, an inhibitor of p44/42 MAPK activation, or SB-203580, an inhibitor of p38 MAPK, did not alter the effect of fibrinogen on fMLP-induced elastase release. Wortmannin, a phosphatidylinositol 3-kinase (PI3K) kinase inhibitor, and genistein, a nonspecific tyrosine kinase inhibitor, strongly inhibited fMLP-induced elastase release both in the presence and in the absence of fibrinogen. An Akt/PKB inhibitor failed to alter the potentiating effect of fi...Continue Reading
References
Jan 1, 1977·The Journal of Experimental Medicine·L A Sherman, J Lee
Oct 1, 1992·European Journal of Pharmacology·K H KrauseD P Lew
Sep 1, 1989·The Journal of Cell Biology·C NathanS D Wright
Apr 15, 1989·European Journal of Biochemistry·R SeifertG Schultz
Aug 25, 1988·Nature·T W SturgillJ L Maller
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S D WrightJ D Loike
Mar 31, 1995·The Journal of Biological Chemistry·J RaingeaudR J Davis
May 1, 1995·The Journal of Experimental Medicine·J D LoikeS C Silverstein
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·G Döring
Oct 1, 1993·European Journal of Haematology·N BorregaardD F Bainton
Dec 1, 1996·Immunity·A CoxonT N Mayadas
Sep 25, 1998·Science·J A Le GoodP J Parker
Oct 9, 1998·The Journal of Biological Chemistry·H M KorchakL E Kilpatrick
Oct 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B WalzogP Gaehtgens
Feb 11, 2000·Archives of Surgery·D A PartrickC C Silliman
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A MócsaiE Ligeti
Aug 24, 2000·Journal of Medicinal Chemistry·Y HuA P Kozikowski
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P M DangA Périanin
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C RubelM S Palermo
Jun 14, 2001·Inflammation·D Granfeldt, C Dahlgren
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H K YumE Abraham
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carolina RubelMarina S Palermo
Jul 31, 2002·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M SaitoM Majima
Nov 14, 2003·American Journal of Physiology. Cell Physiology·John MeshkiSatya P Kunapuli
Nov 15, 2003·Blood·Bryce KerlinHartmut Weiler
Nov 26, 2003·International Immunopharmacology·Florin TulucSatya P Kunapuli
Dec 31, 2003·Experimental Cell Research·Ana Lucia J CoelhoChristina Barja-Fidalgo
Citations
Jan 1, 2005·Indian Journal of Clinical Biochemistry : IJCB·Mohammad Muzaffar MirMohammad Salahuddin
Mar 7, 2006·Pediatric Surgery International·S W Moore
Dec 17, 2009·Cell Biochemistry and Biophysics·Cyril RauchLaurent Counillon
Jan 21, 2011·The Journal of Biological Chemistry·Ya-Ping KoMagnus Höök
Nov 25, 2005·Artificial Organs·Ann Elisabeth Asberg, Vibeke Videm
Mar 7, 2009·Blood·Norma MaugeriAngelo A Manfredi
Aug 19, 2009·European Journal of Pharmacology·Daniel PoeckelOliver Werz
Aug 5, 2008·Journal of Pediatric Surgery·Sam W MooreMonique G Zaahl
Jul 3, 2007·Brain, Behavior, and Immunity·Heike L RittnerShaaban A Mousa
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·J L DegenJ D Goguen
Oct 27, 2004·Medical Hypotheses·Subburaj Kannan
Feb 6, 2014·Journal of Biomedical Materials Research. Part a·Hannah Caitlin CohenW John Kao
Nov 12, 2005·American Journal of Physiology. Cell Physiology·Yu ChenWolfgang G Junger
Oct 10, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Baohua Yang, Victor Rizzo
Oct 28, 2019·Blood Advances·Md Nasimuzzaman, Punam Malik
Mar 4, 2005·Immunity & Ageing : I & a·Anis LarbiTamas Fulop
Jul 28, 2012·Protein & Cell·Ya-Jie XuRong-Qiao He
Jan 13, 2016·Journal of Leukocyte Biology·Kimberly A ThomasLouis B Justement
Jan 30, 2009·Experimental Biology and Medicine·Mani ChopraAvadhesh C Sharma
Nov 10, 2005·The Journal of Biological Chemistry·Yolanda FernándezMaría S Soengas
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derek StrassheimEdward Abraham
Dec 14, 2018·Blood·James P LuyendykMatthew J Flick
Apr 4, 2021·International Journal of Molecular Sciences·Anne-Marije HulshofHugo Ten Cate
Aug 25, 2021·Seminars in Thrombosis and Hemostasis·Katherine J KearneyFraser L Macrae