PMID: 9429306Jun 1, 1997Paper

Primary human muscle satellite cell culture: variations of cell yield, proliferation and differentiation rates according to age and sex of donors, site of muscle biopsy, and delay before processing

Biology of the Cell
S BonavaudG Barlovatz-Meimon

Abstract

The present study was performed to determine the influence on human satellite cell yield, proliferation, and differentiation rates of: 1) sex and age of donors; 2) site of the muscle biopsy; and 3) delay before processing of the muscle biopsy sample. We used a standardized primary muscle cell culture procedure on 206 normal muscle samples obtained from different muscle groups of patients aged from 20 to 88 years, at time of orthopedic surgery. Sex of donors did not influence muscle culture parameters. In contrast, aging tended to affect muscle cell yield (age group 50-59 years vs 70-79 years, P < 0.08), but not myogenic cell abilities to proliferate and to fuse into myotubes. The anatomic origin of muscle samples used for culture appeared to influence culture parameters. In contrast with other tested muscles, the tensor fasciae muscle gave both a good cell yield (174 +/- 25 10(3) cells per gram) and homogeneous proliferation and differentiation rates. Storage of the muscle sample at 4 degrees C in transport medium was associated with a very high cell yield when processing was done in early hours after biopsy (277 +/- 50 10(3) cells/g), a high and stable cell yield when processing was done from day 1 to day 3 after biopsy (185 +...Continue Reading

Citations

Apr 27, 2004·Sports Medicine·Gary R HunterMarcas M Bamman
Nov 13, 2008·Cytotechnology·S BraunA Pavirani
Oct 12, 2004·Arquivos de neuro-psiquiatria·Ana Cristina CamilloRenato Paulo Chopard
Sep 3, 2014·Female Pelvic Medicine & Reconstructive Surgery·Danielle Markle PriceHans Keirstead
Oct 7, 2018·Pflügers Archiv : European journal of physiology·Steven Carter, Thomas P J Solomon
May 24, 2019·Journal of Applied Physiology·Cecilie J L BechshøftAbigail L Mackey
May 4, 2004·Journal of Applied Physiology·Jason C GalleglyEsther E Dupont-Versteegden
Sep 10, 2020·Cytotechnology·Matthew Lee-WingQingdong Guan

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