PMID: 6991512Feb 1, 1980Paper

Primary induction of vitellogenin synthesis in monolayer cultures of amphibian hepatocytes

The Journal of Cell Biology
J E Stanchfield, J D Yager

Abstract

Direct induction of vitellogenin production in cultured male amphibian hepatocytes by estradiol-17 beta has been accomplished. Liver cells were isolated from adult male bullfrogs by collagenase perfusion and maintained as monolayers in serum-free medium containing insulin and estradiol. Vitellogenin production was measured by direct immunoprecipitation from radioactively labeled secreted protein with a specific antiserum against vitellogenin. Significant quantities of vitellogenin were detected in the exported protein on the second day of hormone treatment. Vitellogenin production increased with duration of culture in the presence of estradiol until by the eighth day approximately 90% of secreted protein was vitellogenin. This response is largely comparable to that obtainable in vivo. Indirect immunofluorescence microscopy was used to identify cells synthesizing vitellogenin in response to estradiol. An increase in cytoplasmic fluorescence could be seen in cells throughout the cultures, with increasing time in the presence of estradiol. By the sixth day of treatment, the majority of cells showed significant fluorescence labeling. The results suggest that studies on the mechanisms underlying the primary activation of the vitello...Continue Reading

References

Oct 15, 1978·Experimental Cell Research·J E Stanchfield, J D Yager
Sep 1, 1976·Cell·J R Tata
Jul 12, 1977·Biochemistry·J L ChristmannR C Huang
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A T BurnsR F Goldberger
Aug 1, 1979·Journal of Cellular Physiology·J E Stanchfield, J D Yager
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L J Wangh, J Knowland
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K P MullinixR F Goldberger
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
Jan 1, 1974·Progress in Biophysics and Molecular Biology·M J Clemens

❮ Previous
Next ❯

Citations

Jun 1, 1983·Molecular and Cellular Endocrinology·M P TenniswoodJ R Tata
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C Cayrol, P Deparis
Mar 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·C PelisseroJ P Sumpter
Dec 14, 2006·In Vitro Cellular & Developmental Biology. Animal·Petra-Maja PrelovsekBoris Bulog
Jun 1, 1992·General and Comparative Endocrinology·O Carnevali, G Mosconi
Nov 21, 2001·Toxicology and Applied Pharmacology·A LorenzenT W Moon
May 1, 1988·General and Comparative Endocrinology·C VaillantY Valotaire

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.