Aug 30, 2012

Primary microglia isolation from mixed glial cell cultures of neonatal rat brain tissue

Journal of Visualized Experiments : JoVE
Tami T TamashiroKimberly R Byrnes

Abstract

Microglia account for approximately 12% of the total cellular population in the mammalian brain. While neurons and astrocytes are considered the major cell types of the nervous system, microglia play a significant role in normal brain physiology by monitoring tissue for debris and pathogens and maintaining homeostasis in the parenchyma via phagocytic activity. Microglia are activated during a number of injury and disease conditions, including neurodegenerative disease, traumatic brain injury, and nervous system infection. Under these activating conditions, microglia increase their phagocytic activity, undergo morpohological and proliferative change, and actively secrete reactive oxygen and nitrogen species, pro-inflammatory chemokines and cytokines, often activating a paracrine or autocrine loop. As these microglial responses contribute to disease pathogenesis in neurological conditions, research focused on microglia is warranted. Due to the cellular heterogeneity of the brain, it is technically difficult to obtain sufficient microglial sample material with high purity during in vivo experiments. Current research on the neuroprotective and neurotoxic functions of microglia require a routine technical method to consistently gene...Continue Reading

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Mentioned in this Paper

Brain Injuries
Pathogenic Aspects
Centrifugation, Density Gradient
Pathogenesis
Parenchyma
Cytological Techniques
Infectious Disease of Nervous System
Entire Nervous System
Neurons
Brain

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