PMID: 6104478Mar 1, 1980Paper

Primary monolayer culture of liver parenchymal cells and kidney cortical tubules as a useful new model for biochemical pharmacology and experimental toxicology. Studies in vitro on hepatic membrane transport, induction of liver enzymes, and adaptive changes in renal cortical enzymes

Archives of Toxicology
P Bellemann

Abstract

Freshly isolated liver parenchymal cells were maintained in either short-term monolayer, suspension of long-term monolayer culture. Rapidly occurring processes through hepatocellular membrane, e.g., the enhanced amino acid transport and the concomitantly increased potassium influx following progressive starvation, were kinetically evaluated best in short-term monolayer culture. The inducibility of tyrosine aminotransferase by glucagon, dexamethasone, and a combination of both was compared in suspension and in monolayer culture. The induction of slowly inducible foreign compound-metabolizing enzymes, (e.g., ethoxycoumarin-O-dealkylase, p-nitroanisole-O-demethylase, and UDP-glucuronyltransferase) by phenobarbital, 3-methylcholanthrene, and dexamethasone were studied in long-term monolayer culture. The latter system was also used to maintain isolated kidney cortical tubules for the investigation of renal enzyme adaptation during progressive time in culture.

References

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Citations

Jun 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P H Bach, E N Kwizera
Sep 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D AcostaM A Smith
Jan 1, 1989·Toxicology and Industrial Health·C A Tyson, N H Stacey
Mar 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·I C HsuB F Trump
Jun 1, 1986·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M A SmithJ V Bruckner
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A J Gandolfi, K Brendel
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C A TysonR L Melnick
Feb 1, 1985·Experimental and Molecular Pathology·T A Tran-ThiK Decker

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