Primary neurons become less susceptible to coxsackievirus B5 following maturation: the correlation with the decreased level of CAR expression on cell surface

Journal of Medical Virology
Jeonghyun AhnHeuiran Lee

Abstract

Coxsackievirus B (CVB) is one of the major pathogens of aseptic meningitis and meningioencephalitis, particularly in newborn infants. To analyze the influence of neural maturation on susceptibility to CVB infection, we prepared immature and mature neurons from 16-day-old BALB/c embryonic cortex. In contrast to immature neurons, mature neurons were less susceptible to CVB5 infection, as indicated by the decrease of cytopathic features. In mature neurons, progeny virus production was significantly hindered, and virus capsid protein VP1 synthesis and virus genome amplification were concomitantly reduced. In addition, the expression of coxsackievirus and adenovirus receptor (CAR), the major receptor of CVB5, was down-regulated in mature neurons. The antibody treatment specific to CAR significantly attenuated CVB5 susceptibility of immature neurons. These findings demonstrate that mature neurons become less susceptible to CVB by the decrease of CAR level. Thus, the data strongly support the idea that the level of virus receptor in neurons is one of the crucial determinants in the age-dependency of CVB virulence in central nervous system.

References

Apr 28, 1999·Trends in Microbiology·D E Griffin, J M Hardwick
May 18, 2000·Brain Research. Molecular Brain Research·T HondaR Kuwano
Aug 2, 2001·Reviews in Medical Virology·S D Carson
Mar 29, 2002·Journal of Neurobiology·Christian Lesuisse, Lee J Martin
Jul 4, 2002·Neuroscience Letters·Chul Hyun JooDongHou Kim
Dec 20, 2002·Journal of Neurovirology·John K Fazakerley
Apr 23, 2003·Journal of Neurovirology·Jürgen Schneider-SchauliesSibylle Schneider-Schaulies
May 24, 2003·Brain Research. Developmental Brain Research·Yuko HottaRyozo Kuwano
May 29, 2004·The Journal of General Virology·Jeonghyun AhnHeuiran Lee
Aug 24, 2004·Nature Reviews. Neuroscience·Susanna C Benn, Clifford J Woolf
Sep 15, 2004·The Journal of Biological Chemistry·Carolyn B CoyneJeffrey M Bergelson
Sep 29, 2004·Journal of Virology·Roberta L DeBiasiKenneth L Tyler
Feb 26, 2005·Journal of Virology·Patty S Vernon, Diane E Griffin
Mar 5, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ralph FeuerJ Lindsay Whitton
Jan 21, 2006·Nature Immunology·Taro Kawai, Shizuo Akira
May 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sophie DelhayeThomas Michiels
Jun 30, 2006·Trends in Neurosciences·Anthony N van den Pol
May 24, 2007·Apoptosis : an International Journal on Programmed Cell Death·Nadine JaraschAndreas Henke

❮ Previous
Next ❯

Citations

Jun 29, 2013·Cell Death & Disease·A J KoleM Deshmukh
Nov 28, 2015·Viruses·Hsing-I Huang, Shin-Ru Shih
Jan 22, 2011·Virology·Ross E RhoadesRalph Feuer
Nov 8, 2011·Intervirology·Jenie Yoonoo HwangHeuiran Lee
Dec 18, 2019·Journal of Neuropathology and Experimental Neurology·Waka UshiodaNoriyo Nagata
Mar 11, 2020·Frontiers in Microbiology·Bo-Shiun ChenShin-Ru Shih

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.