PMID: 9174123May 1, 1997Paper

Primary prostatic epithelial cell binding to human bone marrow stroma and the role of alpha2beta1 integrin

Clinical & Experimental Metastasis
S H LangN G Testa

Abstract

Prostate cancer selectively metastasises to the bone. To investigate the importance of prostate epithelial cell adhesion to bone marrow cells in this process we examined the binding of human primary prostatic epithelial cells (PEC) to human bone marrow stromal cultures (BMS). We found that PEC derived from both malignant and benign tissue showed greater adhesion to BMS than to benign prostatic fibroblasts (median difference was 340% and 200% respectively), skin fibroblasts or plastic tissue culture plates. Adhesion to BMS grown from the bone marrow of patients with prostatic skeletal metastases was no different from those grown from normal bone marrow. The role of integrin molecules in these cell interactions was determined. Collagen type I and fibronectin were found to increase PEC adhesion whereas vitronectin and laminin did not. Inhibition studies demonstrated that although there was heterogeneity between samples, antibodies against the integrins alpha2 and beta1 consistently inhibited PEC binding to BMS. This result was more marked for PEC derived from malignant tissue. However studies investigating the effects of disintegrins and anti-alpha3 and anti-alpha5 integrins indicated that for a percentage of patients these integr...Continue Reading

Citations

Jul 18, 2000·Cancer·F W OrrG Singh
Jul 30, 2003·The Journal of Biological Chemistry·Sarmishtha DeTatiana V Byzova
Sep 10, 2005·International Journal of Cancer. Journal International Du Cancer·Tuomas MirttiJyrki Heino
Mar 21, 2007·Journal of Cellular and Molecular Medicine·Denitsa DochevaMatthias Schieker
Feb 26, 2011·European Journal of Nuclear Medicine and Molecular Imaging·Chiun-Wei HuangPeter S Conti
Jul 25, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rupesh I BhattNoel W Clarke
May 20, 2011·Nature Reviews. Cancer·Katherine N WeilbaecherLaurie K McCauley
Jul 21, 2005·Technology in Cancer Research & Treatment·Reetta RiikonenChristian Oker-Blom
Nov 17, 2005·BJU International·Norman J Maitland, Anne Collins
Jan 1, 1999·Journal of Medicinal Food·J HempstockN J George
Dec 4, 2008·Asian Journal of Andrology·Noel W ClarkeMick D Brown
Jul 22, 2011·Journal of Signal Transduction·Moumita ChatterjeeKenneth L van Golen
Nov 15, 2012·Cancer Research·Steven R BarthelCharles J Dimitroff
Sep 21, 2010·Bone·Jochen G SchneiderKatherine N Weilbaecher
Feb 13, 2003·Molecular and Cellular Endocrinology·Xiaoli Shen, Miriam Falzon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.