Primary radical pair P(+)H(-) lifetime in rhodobacter sphaeroides with blocked electron transfer to Q(A). Effect of o-phenanthroline

The Journal of Physical Chemistry. B
Krzysztof Gibasiewicz, Maria Pajzderska

Abstract

Transient absorption spectroscopy with a time resolution of approximately 1 ns was applied to study the decay of the primary radical pair P+H- in Rhodobacter sphaeroides R-26 reaction centers with blocked electron transfer from H- to QA. The block in the electron transfer was realized in two ways: by either reducing or removing QA. We found very different kinetics of the P+H- decay in these two cases. Convolution of the multiexponential decay with the instrument response function allowed resolution of as many as three kinetic components of <1-, 3-4-, and 9-12-ns lifetimes in chromatophores with QA reduced and in isolated reaction centers both with QA either reduced or removed (with or without o-phenanthroline) but with variable relative amplitudes. Removing QA or adding o-phenanthroline to isolated reaction centers increased the amplitude of the slowest decay phase relative to that of the fastest phase. On the basis of these observations, we propose that reaction centers adopt three conformational states characterized by different decay kinetics of P+H-. These conformational states appear to be controlled by the charges in the vicinity of the QA site as revealed by the effects of QA reduction and o-phenanthroline-mediated proto...Continue Reading

References

Mar 31, 1975·Biochimica Et Biophysica Acta·W W Parson, R J Cogdell
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Citations

Jul 3, 2016·Photosynthesis Research·Rafał BiałekKrzysztof Gibasiewicz
Dec 14, 2011·The Journal of Physical Chemistry. B·Haiyu WangNeal W Woodbury
Jul 3, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Krzysztof GibasiewiczMichael R Jones

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