PMID: 2497736Apr 15, 1989Paper

Primary sequence and domain structure of chicken vinculin

The Biochemical Journal
G J PriceDavid R Critchley

Abstract

We have determined the complete sequence of chick vinculin from two overlapping cDNA clones. The vinculin mRNA consists of 262 bp of 5' untranslated sequence, an open reading frame of 3195 bp (excluding the initiation codon) and a long 3' untranslated sequence (greater than 2 kb). Chick vinculin contains 1066 amino acid residues, and has a deduced molecular mass of 116,933 Da. Analysis of the domain structure of vinculin shows that the molecule can be cleaved by V8 proteinase into a 90 kDa globular head and a 32 kDa tail region, the latter of which could further be cleaved into a 27 kDa polypeptide. The 90 kDa globular head contains the N-terminus of vinculin, three 112-residue repeats (residues 259-589), and extends to approximately residue 850. Gel overlay experiments show that it also contains a binding site for the cytoskeletal protein talin. The talin-binding domain was further localized to the N-terminal 398 amino acid residues of the protein by expression in vitro of this region from a vinculin cDNA cloned into the Bluescript SK+ vector. The head and tail domains are apparently separated by a proline-rich region that contains V8-proteinase-cleavage sites and a candidate tyrosine (822)-phosphorylation site. Secondary-stru...Continue Reading

Citations

May 11, 2005·The Journal of Cell Biology·Hui ChenSusan W Craig
Sep 23, 1997·The Journal of Cell Biology·R O LaineF S Southwick
Oct 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M A DeugnierM Glukhova
Jan 1, 1992·Cell Motility and the Cytoskeleton·J L Rodríguez FernándezA Ben-Ze'ev
Jan 8, 2011·Genes, Chromosomes & Cancer·Adrián Mariño-EnríquezAntonio R Pérez-Atayde
Jul 10, 2004·Structure·Robert A BorgonTina Izard
Jun 15, 2004·Nature·Constantina BakolitsaRobert C Liddington
Jan 1, 1990·Cell Motility and the Cytoskeleton·J J Otto
Dec 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F S Southwick, D L Purich
Sep 15, 1992·European Journal of Biochemistry·J V SmallL E Thornell
Nov 7, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·D Michael Shasby
Jan 1, 2013·Protoplasma·Joanne M Leerberg, Alpha S Yap
Feb 1, 1996·Journal of Muscle Research and Cell Motility·W H GoldmannG Isenberg
Aug 18, 1997·FEBS Letters·V E AlatortsevE D Westphal

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