PMID: 7940574Jun 1, 1994Paper

Primary sequence determination of the most basic myonecrotic phospholipase A2 from the venom of Vipera russelli

Toxicon : Official Journal of the International Society on Toxinology
Veerabasappa T GowdaJ L Middlebrook

Abstract

The most basic phospholipase A2 (PLA2), VRV-PL-VIIIa, was purified from (Sri Lankan) Vipera russelli venom. It is a major component of the venom, contributing over 40% to the whole venom PLA2 activity. The purity of VRV-PL-VIIIa was ascertained by electrophoresis and by reverse phase high-pressure liquid-chromatography (RP-HPLC). VRV-PL-VIIIa had an apparent mol. wt of 13,000 and was a single polypeptide. The protein was reduced, pyridylethylated and subjected to sequence analysis. The N-terminal amino acid sequence was established up to the 39th residue. Pyridylethylated VRV-PL-VIIIa was digested with endoprotease Glu-C, and several peptides were purified by RP-HPLC; six purified peptides were sequenced. The sequence of the C-terminal was established by sequencing a CNBr-produced peptide purified by RP-HPLC. Several peptides were also generated by digestion with endoprotease Asp-N. Two peptides were sequenced to obtain overlapping regions. The complete structure was deduced from sequences of overlapping peptides and through homology with other group II PLA2 sequences. Sequence homology was greatest with ammodytoxin A: 99 amino acid residues out of 121 occurred in identical positions. Myotoxin III of Bothrops asper showed 73% h...Continue Reading

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Citations

Aug 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·T V Gowda, J L Middlebrook
Jan 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·I H TsaiJ C Su
Feb 1, 1996·Toxicon : Official Journal of the International Society on Toxinology· Maung-Maung-ThwinC H Tan
Oct 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·B N PrasadT V Gowda
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Lucimara Chioato, R J Ward
Jun 22, 2002·Toxicon : Official Journal of the International Society on Toxinology·Lichuan GuZhengjiong Lin
Oct 9, 2002·Toxicon : Official Journal of the International Society on Toxinology·P S F BarbosaH S A Monteiro
Jan 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·C L OwnbyS P White
Nov 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·H S de AraujoC L Ownby
Mar 10, 2000·Toxicon : Official Journal of the International Society on Toxinology·B Uma, T Veerabasappa Gowda
Sep 15, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Frey Francisco Romero-VargasSergio Marangoni
Jul 11, 2015·Toxicon : Official Journal of the International Society on Toxinology·Maitreyee SharmaRobin Doley
Nov 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Talat JabeenT P Singh
Jun 11, 2014·Analytical Biochemistry·Hamse K VivekS Nanjunda Swamy
Dec 6, 2014·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·J R KumarT V Gowda
Dec 3, 2016·PLoS Neglected Tropical Diseases·Anjana SilvaGeoffrey K Isbister
Oct 12, 2000·Biochemical and Biophysical Research Communications·G IvanovskiJ Pungercar

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