Primary spermatocyte-specific Cre recombinase activity in transgenic mice

Transgenic Research
Sanny S W ChungDebra J Wolgemuth

Abstract

We have evaluated the specificity of Cre recombinase activity in transgenic mice expressing Cre under the control of the synatonemal complex protein 1 (Sycp1) gene promoter. Sycp1Cre mice were crossed with the ROSA26 reporter line R26R, to monitor the male germ cell stage-specificity of Cre activity as well as to verify that Cre was not active previously during development of other tissues. X-gal staining detected Cre-mediated recombination only in testis. Detailed histological examination indicated that weak Cre-mediated recombination occurred as early as in zygotene spermatocytes at stage XI of the cycle of the seminiferous epithelium. Robust expression of X-gal was detected in early to mid-late spermatocytes at stages V-VIII. We conclude that this transgenic line is a powerful tool for deleting genes of interest specifically during male meiosis.

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Citations

Dec 15, 2010·Journal of Cell Science·Manuela PellegriniAndre Nussenzweig
Nov 19, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Lee Smith
Jun 24, 2009·Genesis : the Journal of Genetics and Development·Shirley S Hammond, Angabin Matin
Nov 25, 2006·Biology of Reproduction·Reza J Rasoulpour, Kim Boekelheide
Mar 1, 2012·Molecular Endocrinology·Frédéric PicouFrédéric Flamant

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