PMID: 9426596Jan 14, 1998Paper

Primary structure and expression of acidic (class II) chitinase in potato

Plant Molecular Biology
R BüchterE Kombrink

Abstract

Infection of potato (Solanum tuberosum) leaves by the late blight fungus Phytophthora infestans or treatment with fungal elicitor leads to a strong increase in chitinase activity. We isolated cDNAs encoding acidic (class II) chitinases (ChtA) from potato leaves and determined their structures and expression patterns in healthy and stressed plants. From the total number of cDNAs and the complexity of genomic DNA blots we conclude that acidic chitinase in potato is encoded by a gene family which is considerably smaller than that encoding basic (class I) chitinase (ChtB). The deduced amino acid sequences show 78 to 96% identity to class II chitinases from related plant species tomato, tobacco) whereas the identity to basic chitinases of potato is in the range of 60%. RNA blot analysis revealed that both acidic and basic chitinases were strongly induced by infection or elicitor treatment and that the induction occurred both locally at the site of infection and systemically in upper uninfected leaves. In contrast, a differential response to other types of stress was observed. Acidic chitinase mRNA was strongly induced by salicylic acid, whereas basic chitinase mRNA was induced by ethylene or wounding. In healthy, untreated plants, a...Continue Reading

Citations

Oct 13, 2000·Biological Chemistry·Y PonathE Kombrink
Feb 1, 2005·Proteomics·Valeria DaniRon R D Croy
Jul 17, 2010·Biochemistry. Biokhimii︠a︡·I A TarchevskyA M Egorova
Sep 1, 2005·Molecular Plant Pathology·Marcos MontesanoE Tapio Palva
Aug 7, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Gilda RauscherWilliam E Fry
Jul 30, 2019·Journal of Food Biochemistry·Chen LiZhuanhua Wang
May 21, 2014·Journal of Agricultural and Food Chemistry·Mariateresa VolpicellaLuigi R Ceci
Oct 9, 2021·Frontiers in Bioengineering and Biotechnology·Igor G SinelnikovAleksandra M Rozhkova

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