PMID: 7140761Sep 1, 1982

Primary-structure determination of fourteen neutral oligosaccharides derived from bronchial-mucus glycoproteins of patients suffering from cystic fibrosis, employing 500-MHz 1H-NMR spectroscopy

European Journal of Biochemistry
H van HalbeekP Roussel

Abstract

The structure of carbohydrate units of bronchial-mucus glycoproteins obtained from cystic fibrosis patients was investigated by 500-MHz 1H-NMR spectroscopy and methylation analysis. To that purpose, the mucin was subjected to alkaline borohydride degradation. Neutral oligosaccharide-alditols, ranging in size from disaccharides to pentasaccharides, were isolated. Eight compounds could be purified to homogeneity; furthermore, three fractions were obtained consisting mainly of two components. For all 14 compounds the primary structure could be elucidated. 500-MHz 1H-NMR spectroscopy was found to be effective in detecting heterogeneity and to be invaluable for the determination of structures in mixtures of oligosaccharide-alditols. The structures can be divided into two groups depending on the core disaccharide. One group contains Gal(beta 1 leads to 3)GalNAc-ol as common structural element, the other GlcNAc(beta 1 leads to 3)GalNAc-ol. Both disaccharides were identified as such; the other compounds can be conceived as extensions thereof. The most complex representatives of the two groups are: (formula; see text) The italicized structural elements, comprising the SSEA-1 determinant and the type-1 blood-group-H determinant, are nove...Continue Reading

References

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Citations

Dec 2, 1992·Molecular and Cellular Biochemistry·S SangadalaJ Mendicino
Sep 8, 1993·Molecular and Cellular Biochemistry·S SangadalaJ Mendicino
Dec 2, 2006·Glycoconjugate Journal·Baoyun XiaRichard D Cummings
Nov 30, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·J L GueantJ P Nicolas
Nov 1, 1988·Biochimie·P RousselA Scharfman
May 2, 1984·European Journal of Biochemistry·S A KorrelJ F Vliegenthart
Mar 3, 1986·European Journal of Biochemistry·F G HanischG Uhlenbruck
Apr 15, 1992·European Journal of Biochemistry·F G Hanisch, J Peter-Katalinic
Nov 26, 1999·Biochimica Et Biophysica Acta·T F Scanlin, M C Glick
Jan 1, 1993·Critical Reviews in Clinical Laboratory Sciences·I Brockhausen
Sep 4, 2003·The Journal of Biological Chemistry·Catherine RobbeJean-Claude Michalski
Oct 22, 2013·Expert Review of Respiratory Medicine·Vignesh VenkatakrishnanMorten Thaysen-Andersen
Mar 21, 1998·Biological Chemistry·A V Nieuw AmerongenE C Veerman
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A PrakobpholS J Fisher
Aug 1, 1998·Biological Chemistry·N J ShihA P Jackman

Related Concepts

Pulmonary Cystic Fibrosis
Neoglycoproteins
Hydrogen-Ion Concentration
Mucous Body Substance
In Vivo NMR Spectroscopy
Oligosaccharides

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