Primary structure of human proacrosin deduced from its cDNA sequence

FEBS Letters
T BabaY Arai

Abstract

cDNA clones encoding proacrosin, the zymogen of acrosin, were isolated from a human testis cDNA library by using a fragment of boar acrosin cDNA as a probe. Nucleotide sequencing of the longest cDNA clone has predicted that human proacrosin is synthesized with a 19-amino-acid signal peptide at the N-terminus. The cleavable signal sequence is followed by a 23-residue segment corresponding to the light chain and then by a 379-residue stretch that constitutes the heavy chain containing the catalytic site of the mature protease. The C-terminal portion of the deduced sequence for the heavy chain is very rich in proline residues, most of which are encoded by a unique repeat of CCCCCA. The active-site residues including histidine, aspartic acid, and serine are also predicted to be located at residues 69, 123, and 221, respectively.

References

Jun 1, 1977·European Journal of Biochemistry·B Wiman
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 24, 1977·Biochemical and Biophysical Research Communications·P S Tobias, G F Schumacher
Jun 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W D SchleuningH Fritz
Dec 31, 1985·Biochemistry·D FukushimaS Nakanishi
Dec 21, 1987·FEBS Letters·E Töpfer-Petersen, A Henschen
Aug 25, 1972·Science·L J ZaneveldG F Schumacher
Feb 1, 1974·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W D Schleuning, H Fritz
Nov 1, 1973·European Journal of Biochemistry·E GilboaM Rigbi
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S P LeytusE W Davie
Dec 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·R Fock-NüzelH Fritz

❮ Previous
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Citations

Oct 8, 1991·Biochimica Et Biophysica Acta·U KlemmW Engel
Sep 13, 1994·Biochimica Et Biophysica Acta·R T Richardson, M G O'Rand
Feb 13, 2002·Journal of Reproductive Immunology·Astrid ZahnMonica Hebe Vazquez-Levin
May 31, 1990·European Journal of Biochemistry·S KeimeW Engel
Jul 1, 1992·European Journal of Biochemistry·M H Vazquez-LevinJ W Gordon
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Takumi SasaoMasato Takimoto
Jun 22, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mariola SłowińskaAndrzej Ciereszko
Nov 3, 2011·Andrologia·E Navaeian-KalatM H Nasr-Esfahani
Jul 31, 1992·Biochemical and Biophysical Research Communications·M NakamuraK Arai
Feb 1, 1990·Differentiation; Research in Biological Diversity·U KlemmW Engel
Nov 1, 1991·Archives of Andrology·T KobayashiY Fujimoto
Jul 1, 1991·Archives of Andrology·T KobayashiY Fujimoto
Feb 24, 2000·Biology of Reproduction·L I FurlongM H Vazquez-Levin
Nov 30, 1990·Biochemical and Biophysical Research Communications·S KashiwabaraT Baba
May 15, 1993·The Journal of Experimental Zoology·R M Williams, R Jones
Jul 14, 2005·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Merrilee S Harris, John C Rodger
May 1, 1992·Archives of Andrology·L SilvestroniC Sartori
Mar 22, 2001·The Journal of Biological Chemistry·G W WongR L Stevens
Jan 1, 1990·Andrologia·E Töpfer-PetersenA Zucker
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·H KremlingW Engel
Oct 29, 2020·ACS Infectious Diseases·Anil Mathew TharappelHongmin Li
Jul 14, 2010·Bioorganic & Medicinal Chemistry·M WysockaK Rolka

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Next ❯

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